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《ACTA PARASITOLOGICA ET MEDICA ENTOMOLOGICA SINICA》 1999-03
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CLONING AND SEQUENCING OF TA4 GENE OF EIMERIA TENELLA BJ STRAIN

Li Anxing 1 Qin Zerong 2 Zhang Xinping 2 Huang Qi 3  
TA4 gene of E. tenella BJ strain infecting chickens in Beijing was cloned and sequenced. E. tenella total RNA was extracted from 7h sporulating oocysts and used as a template for cDNA synthesis after reverse transcription. TA4 cDNA was amplified by means of polymerase chain reaction(PCR). The PCR products were checked by agrose gel electrophoresis and then cloned into pGEM T easy Plasmid vector successfully. The TA4 spicific cDNA recombinant plasmids were analysed with Restriction Endonucleases (RE)(HincⅡ、HpaⅠ、NcoⅠ、ScaⅠ、SinⅠ、XmnⅠ and EcoRⅠ)digestion. Nucleotide sequence was determined by dideotide chain termination method. The results indicated that TA4 BJ gene is 1 227bp in lenghth and includes opening read frame which code for a polypeptide of 230 amino acid(molecular weight 25kDa). Comparison of TA4 BJ with the published nucleotide sequence of TA4 demonstrates they have the sequence homologies of 99%. Comparing its deduced amino acid sequence with reported amino acid sequence of TA4 demonstrated that there are 2amino acids changed with Gly from Arp and Leu from Phe.
【Fund】: “8 6 3”青年科学基金
【CateGory Index】: Q785
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