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《Acta Parasitologica Et Medica Entomologica Sinica》 2005-02
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MOLECULAR CHARACTERIZATION OF CONTRACAECUM RUDOLPHII USING RIBOSOMAL ITS-1AND ITS-2 MARKERS

HE Fang WENG Ya-Biao LIN Rui-Qing LI An-Xing ZHU Xing-Quan (College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642)  
The aim of the present study was to assess whether the first(ITS-1)and second(ITS-2)internal transcribed spacers(ITS)of ribosomal DNA(rDNA)could be used as genetic markers for the identification of members of the Contracaecum rudolphii complex.ITS-1 and ITS-2 were amplified by PCR separately from C rudolphii and the amplicons were subjected to single-strand conformation polymorphism(SSCP)analysis,followed by selective sequencing.While no variation in single-stranded ITS-1 and ITS-2 profiles was detected among samples representing each of the species,SSCP analysis of the ITS-1 and ITS-2 amplicons allowed the unequivocal differentiation of all of the three species of Contraceacum examined.Each of the three species had its unique ITS-1 and ITS-2 sequences.The findings of the study supported that C rudolphii comprises two sibling species,C rudolphii A and C rudolphii B.These results indicated that ITS-1 and ITS-2 sequences provide reliable genetic markers for the specific identification of C rudolphill A,C rudolphill B and C.septentrionale.
【Fund】: 国家杰出青年科学基金项目 (3 0 2 2 5 0 3 3 )
【CateGory Index】: R383.1
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