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New Method of Strain Isolation by Magnetic Separation——Strain Isolation by Using Pyrrhotite Culture Medium

Liu Xinxing Xie Jianping Huo Qiang Qiu Guanzhou(Central South University)  
Pyrrhotite can have change in the magnetism due to its different Fe/S.Based on this,they were separated according to their different magnetization intensities by magnetic separation and used as the magnetic culture media for selecting the strains most suitable to grow in culture media with different magnetism.The test results show that the culture medium with strong magnetism may isolate the strain group with short bar shape and particles with no magnetism as the main while the medium with weak magnetism may isolate the strain group with long bar shape and particles with magnetism.The isolated strains can shorten the time for the full oxidation of Fe~(2+) in the culture medium to 30~ 46 hours from 88 hours needed by the original strain.Especially,the isolated strain from the mineral with medium magnetism has a high oxidation activity.In the process of strain isolation,the magnetism of the magnetic culture medium will become weaker gradually due to the bacteria erosion in the mineral crystal lattice.Therefore,to culture the strains appropriate for different magnetism,measures such as changing magnetic particles should be taken in the strain acclimation so as to maintain the magnetism of the culture media.
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