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《Jiangsu Journal of Agricultural Sciences》 2002-01
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Expression of Foreign Genes in Transgenic Plant Progenies of Nonheading Chinese Cabbage

SHEJian ming 1, CAIXiao ning 1, ZHUWei min 1, ZHANGChu xian 1, ZHUZhen 2, YUANXi han 3, SUXiao jun 3, LIBing 3 (1 Institute of Agrobiological Genetics and Physiology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Chin  
Through an agrobacterium mediated transformation,the recombinant plasmids carrying cowpea trypsin inhibitor gene and neomycin phosphotransferase gene were introduced into Brassica campestris ssp chinensis Makino.cv."Zhongjiaoheiye" and "Aijiaoheiye".Insect resistant transgenic plants were obtained in the two varieties.Segregation of kanamycin resistance and insect resistance was observed in T 1 progeny.Three phenotypes:the homozygous line of resistance,heterozygous line of resistance and homozygous line of sensitivity were exhibited in T 2 progeny.The behaviours of kanamycin resistance and insect resistance in progenies of the transgenic plants were conformed to Mendelian Law of the dominant single gene segregation.The homozygous lines of insect resistance were selected from T 2,T 3 and T 4 generation.A number of single plant whose corrected mortalities to the first or second instar larvae of Plutella xylostella (Linnaeus) and Prodenia litura Fabricius reaching 60% were acquired.