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《Jiangsu Journal of Agricultural Sciences》 2008-03
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Micropropagation Technique of Yuhua 2 Peach [Prunus persica(L.) Batsch] in vitro

LI Xiao1,2,YU Ming-liang2,MA Rui-juan2,SHEN Zhi-jun2 (1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Horticulture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)  
Factors influencing explant sterilization,bud induction,proliferation and rooting were studied with the younger stems of Yuhua 2 [Prunus persica(L.) Batsch]in spring.The rapid propagation system was established.Results showed that the best method for sterilization was washing explants with 75.00% alcohol for 20 s and then with 0.10% mercuric chloride + 0.01% tween for 8 min.The optimal medium for inducing axillary bud was LP+6-BA 0.80 mg/L+IBA 0.30 mg/L;for bud proliferation was LP+6-BA 1.50 mg/L+IBA 0.20 mg/L,in which the plant was stronger and the proliferation rate reached 3.5-4.0 times in 4 weeks;the optimal medium for rooting was MS +IAA 1.50 mg/L.We also found that after adding 0.20 mg/L GA3 in the medium the yellow plant reverted green.LP medium supplemented with 0.729 mmol/L Ca2+ or 6.50 g/L agar also can control vitrification.
【Fund】: 国家科技基础条件平台项目(2005DKA21002-21);; 农业行业科研专项(nyhyzx07-025)
【CateGory Index】: S662.1
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