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《Jiangsu Journal of Agricultural Sciences》 2009-03
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Extraction of Genomic DNA from Acer yangjuechi and System Optimization of SRAP-PCR

LI Qian-zhong1,LIU Xiao-hong2,SU Jia-le1,CONG Yu1(1.Initute of Horticulture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.College of Agriculture,Yangzhou University,Yangzhou 225009,China)  
To find a rapid and effective DNA extraction method for Acer,CTAB method,SDS method and Na2S2O5 method were compared.The DNA was detected by Eppdendorf Biophotometer and agarosegel electrophoresis.The results showed the CTAB method was much better than the others in extracting DNA of Acer yangjuechi,and which was suitable for SRAP-PCR analysis.The concentrations of dNTPs,Mg2+ and primers which affect the SRAP-PCR reactions were optimized in order to establish the SRAP molecular marker system in Acer.The optimum system was as follows: template DNA 100 ng,10×PCR Buffer(Mg2+ free),Mg2+ 2.5 mmol/L,dNTPs 0.2 mmol/L,primers 0.5 μmol/L,Taq DNA polymerase 1.0 U.The total volume of reaction was 50 μl.
【Fund】: 江苏省农业科技自主创新基金项目[CX(07)610];; 江苏省林业三项工程项目[lysx(2007)16]
【CateGory Index】: Q943.2
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【Citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Liwang et al(Department of Horticulture,Nanjing Agricultural University,210095);Genomic DNA Extraction and RAPD Analysis of Eggplant and Its Wild Relatives[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1997-03
2 LI Shan,WANG Ma li,ZHAO Gui fang * (Institute of Life Science,Biodiversity Research Center of Qinling Mts.,Northwest University,Xian 710069,China);DNA extraction and characterization of Acer miaotaiense[J];Acta Botanica Boreali-occidentalia Sinica;2001-02
3 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
4 Wu Xin 1,2,3 Lei Tiangang 2,3 He Yongrui 2,3 Liu Xiaofeng 2,3 Xu Lanzhen 2,3 Peng Aihong 2,3 Chen Shanchun 2,3 1 College of Horticulture & Landscape Designing, Southwest University, Chongqing, 400716; 2 Citrus Research Institute, Southwest University, Chongqing, 400712; 3 National Citrus Cultivar Improvement Center of China, Chongqing, 400712;Establishment and Optimization of SRAP and ISSR Marker System in Citrus[J];Molecular Plant Breeding;2008-01
5 GUO Da-long, LUO Zheng-rong*(Key Laboratory For Horticultural Plant Biology, MOE, Huazhong Agricultural University, Wuhan, Hubei 430070 China);Optimization of SRAP -PCR in some Diospyros spp.[J];Journal of Fruit Science;2006-01
6 WANG Yong-kang, WANG Yong-qin, TIAN Jian-bao, FAN Xin-ping,LI Deng-ke, SUI Chuan-ling, HUANG Cong-lin2(1Rresearch Institute of Pomology, Shanxi Academy of Agricultural Science, Taigu, Shanxi 030815 China; Beijing Agro-Biotechnology Research Center, Beijing100089 China);Extraction methods of high quality DNA from Zizyphus jujuba[J];Journal of Fruit Science;2006-02
7 LI Yuan-yuan, DAI Hong-yan, GUO Xiu-wu*, LIU Hai-tao, CHANG Lin-lin(College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110161 China);Comparison of the total DNA extraction methods in hawthorn[J];Journal of Fruit Science;2007-01
8 QIAO Yan-chun, LIN Shun-quan*, LIU Cheng-ming, YANG Xiang-hui (College of Horticulture,South China Agricultural University, Guangzhou, Guangdong 510642 China);Optimization of SRAP analysis and its application in germplasm research of Loquat (Eriobotrya japonica)[J];Journal of Fruit Science;2008-03
9 Li Niya1, Wang Fang1, Shen Wentao2, Li Dazhou3 (1. Department of Biology,Hainan Normal University,Haikou 571158,China; 2. Institute of Tropical Bioscience and Biotechnology, CATAS,Haikou 571101,China; 3. Institute of Hainan Foresty Research,Haikou 571100,China);Comparison of coconut genome DNA extracting methods[J];Journal of Hainan Normal University(Natural Science);2008-01
10 Ma Xihan Wang Lanzhen Wang Shuqing (Dept. of Basic Courses NWFC,Yangling, Shaanxi, 712100);Advances in the Researches of the Chemical Constituents of Aceraceous Plants[J];JOURNAL OF NORTHWEST FORESTRY COLLEGE;1998-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 MA Rong-qun,SONG Zheng-xu,HUANG Yue,YUE Wen-hui*(Qingdao Institute of Agricultural Sciences,Qingdao 266100,China);Application Progress of Modern Biotechnology on Genetic Research of Welsh Onion(Allium fistulosum L.)[J];Shandong Agricultural Sciences;2009-06
2 Liu Liwang et al (National Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture, Nanjing Agriculture University, Nanjing,Jiangsu 210095);Advance in Eggplant Genetic Breeding with Biotechnology[J];Journal of Anhui Agricultural Sciences;2002-03
3 LIU Zun-chun et al(Henan Institute of Science and Technology,Xinxiang,Henan 453003);Comparison of Genomic DNA Extraction Methods of Jinhua Pear[J];Journal of Anhui Agricultural Sciences;2005-11
4 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
5 WANG Min-qiang et al(College of Chemistry,Engineering and Biological Science,Yantai University,Yantai,Shandong 264005);Effect of Three Anticoagulants on Chicken's Blood and the Quality of DNA Extraction[J];Journal of Anhui Agricultural Sciences;2006-23
6 JIA Bing et al(The Key Laboratory of Pomology,Anhui Agricultural University,Hefei,Anhui 230036);Study on the Different Extraction Method of Genomic DNA of Juicy Peach Stem-tip[J];Journal of Anhui Agricultural Sciences;2007-06
7 ZHAO Xin-liang et al (Northwest A&F University,Yangling,Shaanxi 712100);Study on Classification of Maize Inbred Lines in Henan with SRAP Marker Technology[J];Journal of Anhui Agricultural Sciences;2007-07
8 YI Qing-ping et al (Department of Bio-engineering,Jingchu College of Technology,Jingmen,Hubei 448200);Advances of Genome DNA Extraction and Purification in Plants[J];Journal of Anhui Agricultural Sciences;2007-25
9 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
10 DING Jin-ping et al (College of Agronomy,Guangxi University,Nanning,Guangxi 530003);Establishment and Optimization of SRAP Reaction Conditions for Arachis hypogaea L.[J];Journal of Anhui Agricultural Sciences;2008-10
China Proceedings of conference Full-text Database 10 Hits
1 Fang Jinggui, Qiao Yushan, Gao Zhihong, Wang Sanhong (Department of Horticulture, Nanjing Agricultural University, Nanjing 210095);The Utilization of DNA Presen'ation in the Conservationof Fruit Germplasm Resources[A];[C];2000
2 Huang Daiqing, Lu Liuxin ( College of Horticulture , Fujian Agriculture and Forestry University, Fuzhou, 350002; College of Bio - engineering, Fujian Normal University, Fuzhou , 350007);PCR-based Cloning and Analysis of NBS-LRR Class Resistance Gene Analogs in Five Fruit Trees[A];[C];2002
3 Liu Chengming and Mei Mantong ( College of Horticulture , Genetic Engineering Laboratory, College of Life Scicence , South China Agricultural University, Guangzhou 510642);Researches on DNA isolation and Optimal Parameters of RAPD in Some Sapindaceae Fruit Trees[A];[C];2002
4 REN Yu YANG Guang-sui YIN Jun-mei (Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agriculture Sciences,Danzhou 571737);Analysis of Genetic Diversity in Dendrobium Germplasm by SRAP Makers[A];[C];2007
5 ;Cultivar Identification and Fingerprinting of Eggplant (Solanum melongena L.) with Molecular Markers[A];[C];2008
6 ;The Principle and Research Advancement of Sequence-Related Amplified Polymorphism[A];[C];2008
7 XIE Zong-ming~1 DONG yong-mei~1 SU Jun-ji~2 (1.Center for Molecular Agrobiotechnology and Breeding,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China; 2.Xinjiang;Cotton Research Institute,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi Xinjiang 832000,China);Methods and Techniques Used to Test Genuiness and Purity of Seed of Hybrid Upland Cotton[A];[C];2008
8 Liu dan;Qiao yushan;Nie yun;Shen zhijun;Yuan weiming;Zhang zhen College of Horticulture Nanjing Agricultural University, Nanjing 210095 Institust of evergreen fruit of Taihu in Jiangsu province, Suzhou 215107;Identification of Genetic Variation of Loquat Using Inter Simple Sequence Repeat[A];[C];2003
9 Jianming GAO,Zhongyou PEI,Qiuling CHEN,Jinzhao WEI,Zifang Li Feng LUO,Bo DING,Shoujun SUN* (Department of Agronomy,Tianjin Agricultural University,Tianjin 300384,P.R.China;;AFLP and SRAP fingerprinting of a collection of sweet sorghum germplasm[A];[C];2008
10 Chen Hui-Yun, Sun Zhi-Dong, Mao Yi-Jun, Wang Xiao-Wu, Ge Hong) (Ningbo Academy of Agricultural Sciences, Ningbo, China. 315040. China Academy of Agricultural Sciences, vegetable and flower institute, Beijing, china. 100081);Study on isolation of genome DNA in Cymbidium goeringii. Rchb.f.[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Dong-mei et al (Shanghai Landscape Gardening Research Institute,Shanghai 200232);Study on the Extraction of Anthocyanin of Acer platanoides and its Stability[J];Journal of Anhui Agricultural Sciences;2006-13
2 Liu Liwang et al(Department of Horticulture,Nanjing Agricultural University,210095);Genomic DNA Extraction and RAPD Analysis of Eggplant and Its Wild Relatives[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1997-03
3 QI Zhen-sheng~1,LU Gui_qiao~(1,2),GU Jian-cai~(1,2),YANG Hua-sheng~1(1.Agricultural University of Hebei,Baoding 071000,P.R.China;2.College of Soil and Water Conservation,Beijing Forestry University,100083,P.R.China);Textual Researches on the Wrong Spread of Feng(枫) and the Wrong Correction Made for Qi(槭)[J];Journal of Beijing Forestry University(Social Sciences);2006-02
4 MA Jin WANG Xiao-de (Faculty of Landscape Architecture and Art,Zhejiang Forestry College,Lin'an,Zhejiang 311300,China);The Germplasm Resource of Maple Species and Assessment of Their Exploitations and Utilization in Tianmu Mountain[J];Journal of Yangtze University(Natural Science Edition)Agricultural Science Volume;2005-02
5 LI Shan,WANG Ma li,ZHAO Gui fang * (Institute of Life Science,Biodiversity Research Center of Qinling Mts.,Northwest University,Xian 710069,China);DNA extraction and characterization of Acer miaotaiense[J];Acta Botanica Boreali-occidentalia Sinica;2001-02
6 YANG Juan~1,LI Shan~2,CAO Dong-wei~1,LIU Zhan-lin~1,ZHAO Gui-fang~(1*)(1 Biodiversity Research Center of Mts.Qinling,Northwest University,Xi'an 710069,China;2 College of Life Sciences and Technology,Tongji University,Shanghai 200092,China);Construction and Analysis of the AFLP Reaction System of Dipteronia Oliv.[J];Acta Botanica Boreali-occidentalia Sinica;2005-11
7 WANG Xiao-ming, LIANG Jia-sheng ,MIAO Ru-huai (City Administration Bureau of Shenzhen,Shenzhen,Guangdong 518049,China;School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China);A New Species of Acer (Aceraceae) from Southern China[J];Acta Botanica Boreali-Occidentalia Sinica;2006-04
8 Zhang Wencheng (Northwestern Institute of Botony,Academia sinica,Yangling,Shaanxi 712100);STUDY ON CHEMICAL CONSTITUENTS IN SEEDS OF DIPTERONIA SINENSIS OLIV. NATIVE TO CHINA[J];Acta Botanica Boreali-occidentalia Sinica;1994-05
9 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
10 GUO Da-long, LUO Zheng-rong*(Key Laboratory For Horticultural Plant Biology, MOE, Huazhong Agricultural University, Wuhan, Hubei 430070 China);Optimization of SRAP -PCR in some Diospyros spp.[J];Journal of Fruit Science;2006-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Yue-guang~(1*),TENG-Yong yong~(3*),PAN Chen~1,HAN Yan-chuang~1,ZHOU Ming-quan~2,HU Zhong-li~(1,2**)(1 Key Lab of the Ministry of Education for Plant Developmental Biology,Wuhan University,Wuhan 430072;2 Lotus center,Wuhan University,Wuhan 430072;3 College of Life Sciellce,Xinyang Nomal University,Xinyang 46400,China);Cluster Analysis of Nelumbo Based on SRAP Markers[J];Amino Acids & Biotic Resources;2006-01
2 MAO Yong min 1,LU Jin ying 2,SHEN Lian ying 1,DAI Li 1,LIU Ping 1,PENG Shi qi 1 (1. Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001,China; 2. Department of Horticulture, Agricultural University of Hebei, Ba;The effects of vesicular-arbuscular mycorrhizal fungi on growth of wild jujube (Zizyphus spinosus Hu) seedlings[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;2000-02
3 PENG Sa~(1,2),GUO Mei-li~(1*),CHEN Yue-hua~(2,3),GUO Qing-hua~(1)(1.Department of Pharmacognosy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China;2.College of Agronomy,Xinjiang Agricultural University,Urumchi 830000;3.Institute of Economic Crop,Xinjiang Academy of Agricultural Sciences,Urumchi 830000);Establishment and optimization of sequence-related amplified polymorphism amplification system for Carthamus tinctorius L.[J];Academic Journal of Second Military Medical University;2006-05
4 WANG Ting 1,SU Ying juan 1,HUANG Chao 2,ZHU Jian ming 1\j(1 School of Life Sciences,Zhongshan University,Guangzhou 510275,China;2 Department of Pharmacy,Hubei Chinese Traditional Medicine College,Wuhan 430061,China);Phylogenetic relationships of taxaceae based on random amplified polymorphic DNA[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;2000-02
5 LI Ni ya 1,GAO Pei yuan 2,ZHOU Peng 3,GUO An ping 3,ZHONG Qiong xin 1 (1 Department of Biology,Hainan Normal University,Haikou 571158,China;2 Tropical Plant Tissue Culture Center of Hainan Province,Haikou 570314,China;3 National Key Biotechnology Laboratory for Tropical Crops,CATAS,Haikou 571101,China);Studies on identification and classification of genomic DNA in Aloe by RAPD analysis[J];Acta Botanica Boreali-occidentalia Sinica;2002-06
6 Chen Yongjiu Zhang Yaping (Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223);STUDIES ON FACTORS IN RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS[J];ZOOLOGICAL RESEARCH;1997-02
7 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
8 Lei Tiangang1,2,3 He Yongrui1,2,3 Wu Xin2 Liu Xiaofeng1,2,3 Xu Lanzhen1,2,3 Peng Aihong1,2,3 Chen Shanchun1,2,3 1 Citrus Research Institute,CAAS,Chongqing,400712;2 Citrus Research Institute,Southwest University,Chongqing,400712;3 National Citrus Cultivar Improvement Center of China,Chongqing,400712;Identification of Pummelo (Citrus grandis (L.) Osbeck) and Hybrid Citrus Cultivars using SSR Molecular Markers[J];Molecular Plant Breeding;2007-05
9 Jin Yongfeng;Zhang Yaozhoul;Chen Daming and Zhang Shanglong (Institute of Biochemistry, Department of Horticulture, Zhejiang Agricultural University, Hangzhon 310029);Identification of Peach Early-ripening Mutant 'Daguang 1' by RAPD Markers and Cloning of Specific Fragment[J];JOURNAL OF FRUIT SCIENCE;1998-02
10 Xuan Jiping,Zhang Zhen ,Fang Jinggui ,Gao Zhihong,and Che Shengli (College of Horticulture,Nanjing Agricutural University,Nanjing,Jiangsu210095China);Construction of Apple Ccultivar IS SR Fingerprinting[J];Journal of Fruit Science;2002-06
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