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《Jiangsu Journal of Agricultural Sciences》 2009-03
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Optimization of SRAP System and Cluster Analysis of Non-heading Chinese Cabbage (Brassica campestris L. ssp. chinensis)

SHAN Xiao-zheng1,HOU Xi-lin 1,2,LI Ying1,2,WU Hai-tao1,WANG Feng1,LI Mei1(1.National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China;2.Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing 210095,China)  
The suitable concentration of four factors,Mg2+,dNTP,Taq polymerase,and primer were identified by single factor tests in the SRAP amplification system on non-heading cabbage(Brassica campestris L.ssp.Chinensis),and orthogonal scheme in four factors at three levels was designed based on the result of above single factor test to optimize SRAP amplification system.An ideally SRAP reaction system was established which included 25 μl reaction volume containing Mg2+ 2 mmol/L,dNTP 0.2 mmol/L,Taq polymerase 1.5 U,primer 0.36 μmol/L,1×PCR buffer,and template DNA 30 ng.The molecular identification of 17 non-heading Chinese cabbage cultivars was conducted with the optimized SRAP marker system.20 out of 30 primer combinations used could detect 90 polymorphic loci.Cluster analysis(UPGMA) showed the cultivars that share similar horticultural trait was clustered at a lower genetic distance,which indicated that SRAP would play an important role in non-heading cabbage identification and cluster analysis.
【Fund】: 上海市重大科技攻关项目(06DZ19103);; 新世纪优秀人才支持计划项目(NCET-07-0439)
【CateGory Index】: S634.302
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【References】
Chinese Journal Full-text Database 8 Hits
1 HOU Yu-rong,AN Sha-zhou,LIU Dong,WANG Wei,XU Cai-qing(College of Grassland and Environment Science,Xinjiang Agricultural University;Key Laboratory of Grassland Resources and Ecology of Xinjiang,Xinjiang Urumqi 830052,China);Influence of differently geographical populations and terrains on genetic diversity of Seriphidium transiliense[J];Pratacultural Science;2011-01
2 CHEN Hui1,2,CHEN Mei-xia1,3,CHEN Yan-ping1,4,WEI Cheng-lin1,LIAO Ying-min1,CHEN Fu-cheng1, TAO Ai-fen1,XU Jian-tang1,QI Jian-min1(1.Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;2.Dongshan Entry-Exit Inspection and Quarantine Bureau,Zhangzhou,Fujian 363401,China;3.Ningde Normal University Biological Engineering,Ningde,Fujian 352100,China;4.Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China);Extraction of Genomic DNA from Corohorus olitorius L. and Establishment of SRAP Reaction System[J];Fujian Journal of Agricultural Sciences;2011-05
3 Zhong Kaiqin Zhu Chaohui Chen Wenhui Fang Shugui Lin Hefei Fuzhou Institute of Vegetable Science, Fuzhou, 350111;Purity Identification of Cauliflower ‘Songhua 55 day’ Using SRAP Markers[J];Molecular Plant Breeding;2011-06
4 WANG Cong-yan(School of Life Sciences,Nantong University,Nantong 226019,China);Research Progresses of SRAP Application on Plants in Genetic Diversities[J];Biotechnology;2011-05
5 Jing Zange Tang Zheng Zhang Xiaoling Luo Tiankuan Liu QingZhu Shiyang Lou Jue Ye Zhen Chen Haiying(Zhenan Key Laboratory of Crop Breeding,Biotechnology Research Institution,Wenzhou Vocational College of Science and Technology,Wenzhou 325006);Optimization of SRAP-PCR System and Cultivar Molecular Identification in Broccoli(Brassica oleracea var. italica)[J];Biotechnology Bulletin;2010-12
6 HUANG Jian-du, SHAO Gui-rong, CHEN Wen-hui, FANG Shu-gui, ZHONG Kai-qin (Fuzhou Vegetable Research Institute, Fuzhou, Fujian, 350111, China);Genetic Purity Test of Cabbage Xiahua No. 2 Using SRAP Markers[J];China Cucurbits and Vegetables;2012-02
7 Jiang Haikun,Wang Mingxia,Yan Congsheng,Wang Yan,Ma Shaojun,Dong Yanxiang,Fang Ling,Zhang Qi ' an(Institute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei 230031);Optimization of SRAP Reaction System on Mini-watermelon[J];Chinese Agricultural Science Bulletin;2011-28
8 WANG Ming-xia, YAN Cong-sheng, JIANG Hai-kun, MA Shao-jun, FANG Ling (Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031, China);Optimization of SRAP-PCR Reaction for Brassica campestris L. ssp. Chinese(L.) Makino var. rosularis Tsen et Lee.[J];China Cucurbits and Vegetables;2013-02
China Proceedings of conference Full-text Database 1 Hits
1 WANG Ming-xia,YAN Cong-sheng,JIANG Hai-kun,MA Shao-jun,and FANG Ling (Institute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei 230031);Optimization of SRAP Reaction System on Brassica campestris L.ssp.Chinese(L.) Makino var rosularis Tsen et Lee.in Anhui Province[A];[C];2012
【Citations】
Chinese Journal Full-text Database 10 Hits
1 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
2 Wang Jia Liang Guohua Miao Minmin Chen Xuehao*Key Laboratory of Education Ministry for Plant Function Genomics, Yangzhou University, Yangzhou, 225009;Optimization for ISSR Reaction System in Cucumis sativus L. Using Orthogonal Design[J];Molecular Plant Breeding;2006-03
3 Liu Lijun Meng Zuqing Xing Xiulong Peng Dingxiang* College of Plant Science &Technology, Huazhong Agricultural University, Wuhan, 430070;Optimization for SRAP Reaction System in Ramie (Boehmeria nivea L. Guad)[J];Molecular Plant Breeding;2006-05
4 Chen Wansheng Wang Yuanying Luo Chenggang Yang Aiguo Jang Caihong Fan Jingyuan Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, 266101;Optimization for SRAP-PCR System of Tobacoo Based on Orthogonal Design[J];Molecular Plant Breeding;2008-01
5 CHEN Feng,ZHANG Jie-fu,CHEN Song,GU Hui,QI Cun-kou(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);SRAP Markers Linked to Recessive Genic Male Sterile Gene in Rapeseed(Brassica napus L.)[J];Jiangsu Journal of Agricultural Sciences;2007-04
6 Cao Shou-chun;Li Shi-jun;A PRELIMINARY STUDY ON THE LOCAL VARIETY OF CHINESE CABBAGE(BRASSICA CHINENSIS L.)I.MORPHOLOGICAL OBSERVATION AND STUDY[J];Journal of Nanjing Agricultural University;1980-02
7 Cao Shouchun,Li Shijun;A PRELIMINARY STUDY OF THE LOCAL VARIETIES OF CHINESE CABBAGE (BRASSICA CHINENSIS L. )——Ⅲ. CLASSIFICATION OF CULTIVARS.OF NONHEADING CHINESE CABBAGE[J];Journal of Nanjing Agricultural University;1982-02
8 HOU Xi-lin (National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agric Univ, Nanjing 210095,China);Advances in breeding of non-heading Chinese cabbage[J];Journal of Nanjing Agricultural University;2003-04
9 LIN Ping~1,ZHANG Han-guo~2,XIE Yun-hai~2(1.Forestry Research Institute of Heilongjiang Province,Harbin 150040;2.Department of Forestry,Northeast Forestry University,Harbin 150040,China);Study on Optimization for ISSR Reaction System of Larix using Orthogonal Design[J];Biotechnology;2005-05
10 YANG Qi and ZHANG Lu-gang (College of Horticulture,Northwest A & F University,Yangling Shaanxi 712100,China);Optimization of SRAP Reaction System in Chinese Cabbage(Brassica campestris syn. rapa L. ssp. Pekinensis(Lour) olsson)[J];Acta Agriculturae Boreali-Occidentalis Sinica;2007-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 MU Jin-hua,XU Wen-ling,ZHANG Yi-hui,WANG Cui-hua(Vegetable Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Screening Resistance to Chinese Cabbage Soft Rot by Molecular Marker[J];Shandong Agricultural Sciences;2008-04
2 CHEN Jing,HU Xiao-hui,MIAO Hua-rong,ZHANG Jian-cheng,YU Shan-lin(Shandong Peanut Research Institute,Qingdao 266100,China);Optimization of SRAP-PCR Reaction System on Peanut[J];Shandong Agricultural Sciences;2008-05
3 MA Rong-qun,SONG Zheng-xu,HUANG Yue,YUE Wen-hui*(Qingdao Institute of Agricultural Sciences,Qingdao 266100,China);Application Progress of Modern Biotechnology on Genetic Research of Welsh Onion(Allium fistulosum L.)[J];Shandong Agricultural Sciences;2009-06
4 HUO Yu-meng,MIAO Jun,YANG Yan-yan,LIU Bing-jiang,ZHANG Yi-hui,WU Xiong,HE Qi-wei(Vegetable Research Institute,Shandong Academy of Agricultural Sciences/ Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province/ Shandong Branch of National Center for Vegetable Improvement,Jinan 250100,China);Optimization of SRAP-PCR System for Onion[J];Shandong Agricultural Sciences;2011-04
5 Huang Lei et al. (Life Science College of Anhui science and Technology University,Fengyang 233100,China);Advance on the Study of Application of a Novel Molecular Marker[J];Anhui Agricultural Science Bulletin;2010-23
6 Zhao Yao et al.(Lu zhou City Agriculture Bureau,Luzhou 646000,China);The Application of SSR Markers in Seeds Quality Detection[J];Anhui Agricultural Science Bulletin;2011-07
7 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
8 GUAN De-jun (Depatrment of Life Science,Anqing Normal University,Anqing,Anhui 246011);Principle of AFLP and its Application in Plant[J];Journal of Anhui Agricultural Sciences;2006-15
9 WANG Jia et al(College of Landscape Architecture,Beijing Forestry University,Beijing 100083);Study on Optimization for ISSR Reaction System of Peony with Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2006-24
10 LIU Qing-shen et al(College of Animal Science,South China Agricultural University,Guangzhou,Guangdong 510642);Preliminary Study on the Genetic Background of Chinese Shar-pei in its Origin Place with RAPD Technique[J];Journal of Anhui Agricultural Sciences;2007-03
China Proceedings of conference Full-text Database 10 Hits
1 SHI Qian-qian WANG Yan ZHOU Lin (Research Institute of Forestry,CAF,SFA Key of Breeding and Cultivation,Beijing 100091);Optimization of SRAP-PCR System and Selection of Primers for Paeonia suffruticosa Based on Capillary Electrophoresis[A];[C];2011
2 YU Cui,LIU Chun~*,MING Jun,and YUAN Su-xia (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing,100081);Establishment and Optimization of SRAP-PCR Reaction System on Anthurium andraeanum[A];[C];2011
3 Shi Gongjun, Hou Xilin, and Wang Yanhua (National Key Laboratory of Crop Genetics & Germplasm Enhancement, College of horticulture, Nanjing Agricultural University, Nanjing, 210095 China);Cloning and Sequence Analysis of cDNA Fragment of atp6 Gene Related to Cytoplasmic Male Sterility in Non-heading Chinese Cabbage[A];[C];2004
4 Zhao Jianjun Wang Xiaowu Guusje.Bonnema Dick.Vreugdenhil Maarten.Koornneef Xu Zeyong Sun Rifen (Institute of Vegetables and Flowers, Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, College of Life Science, Hebei Agricultural University ; Laboratory of Plant Genetics and Breeding, Wageningen University, The Netherlands);Genetic Diversity for Brassica rapa Inferred from AFLP Fingerprints[A];[C];2004
5 REN Yu YANG Guang-sui YIN Jun-mei (Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agriculture Sciences,Danzhou 571737);Analysis of Genetic Diversity in Dendrobium Germplasm by SRAP Makers[A];[C];2007
6 ;SRAP Molecular Marker Related to Green Peel Gene in Cucumber[A];[C];2008
7 ;An ISSR Marker Linked to the Dwarf Gene of Cucumber[A];[C];2008
8 ;Cultivar Identification and Fingerprinting of Eggplant (Solanum melongena L.) with Molecular Markers[A];[C];2008
9 ;The Principle and Research Advancement of Sequence-Related Amplified Polymorphism[A];[C];2008
10 ZUO Dan-dan MING Jun LIU Chun MU Ding(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081);Paternity Analysis of Chimonanthus praecox (L.) Link Using Sequence-Related Amplified Polymorphism Marker[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Shu-xia,SUN Zhao-fa,SONG Chao-yu(Qingdao Academy of Agricultural Sciences,Qingdao 266100,China);Comparative Test on Premature Chinese Cabbage Varieties with Heat Tolerance[J];Shandong Agricultural Sciences;2009-01
2 WANG Yan-chun et al (Deparment of Grassland Science,Sichuan Agricultural University,Yaan,Sichuan 625014);Analysis of the Characteristic and Element of Criterion for Soil Degradation in the Sub-alpine Meadow[J];Journal of Anhui Agricultural Sciences;2006-13
3 ZHANG Li-hui et al (Department of Biology,Changchun Teachers College,Changchun,Jilin 130032);Quantitative Character of Puccinellia tenuiflora and Puccinellia chinampoensis and the Relationship between the Character and the Clone Size of Two Puccinelila Populations at Reviving Stage in the Songnen Plain[J];Journal of Anhui Agricultural Sciences;2006-21
4 TAN Tan et al(College of Natural Conservation,Bejing Forestry University,Beijing 100083);Optimization of the ISSR-PCR Reaction System of Cyclocarya paliurus[J];Journal of Anhui Agricultural Sciences;2006-24
5 SHI Jian-wei et al(Institute of Loess Plateau,Shanxi University,Taiyuan,Shanxi 030006);Effect of Eenvironmental Stress on Non-structural Carbohydrates in Plant Body[J];Journal of Anhui Agricultural Sciences;2007-09
6 LI Xiao-hui et al(Institute of Horticulture,Henan Academy of Agriculture sciences,Zhengzhou,Henan 450002);Optimization of SRAP-PCR Program and System in Citrullus lanatus[J];Journal of Anhui Agricultural Sciences;2007-27
7 YIN Ji-yan et al (The Services Command College of Chinese People's Armed Police Force,Beijing 102202);Several Common Genetic Markers Methods Applied in the Research of Genetic Evolution[J];Journal of Anhui Agricultural Sciences;2007-34
8 LU Hong-mei et al(Guizhou Provincal Key Laboratory of Fermentation Engineering and Biopharmacy,Guiyang,Guizhou 550003);Study on Changes of Soil Chemical Properties in the Process of Karst Rocky Desertification[J];Journal of Anhui Agricultural Sciences;2009-28
9 DENG Bo-tao ZHAO Fei WANG Xue-ying (College of Biological Sciences and Technology,Shenyang Agricultural University,Shenyang Liaoning 110161);Optimization of SRAP in Carrot[J];;2009-13
10 ZHANG Lin~1,2,3; LUO Tian-xiang~2; DENG Kun-mei~1; LI Wen-hua~1.1 Institute of Geographic Sciences and Natural Resources Research,Chinese Academy of Sciences,Beijing,100101,P. R. China;2 Institute of Tibetan Plateau,Chinese Academy of Sciences,Beijing,100085,P. R. China;3 Graduate University of Chinese Academy of Sciences,Beijing,100049,P. R. China.;Vertical variations in specific leaf area and leaf dry matter content with canopy height in Pinus yunnanensis[J];Journal of Beijing Forestry University;2008-01
China Proceedings of conference Full-text Database 1 Hits
1 Zhao Jianjun Wang Xiaowu Guusje.Bonnema Dick.Vreugdenhil Maarten.Koornneef Xu Zeyong Sun Rifen (Institute of Vegetables and Flowers, Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, College of Life Science, Hebei Agricultural University ; Laboratory of Plant Genetics and Breeding, Wageningen University, The Netherlands);Genetic Diversity for Brassica rapa Inferred from AFLP Fingerprints[A];[C];2004
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Hai-li et al(Wenzhou Academy of Agricultural Sciences,Wenzhou,Zhejiang 325014);SRAP Analysis on the Molecular Fingerprint of Tomato Cultivars[J];Journal of Anhui Agricultural Sciences;2012-16
2 Zhong Kaiqin Zhu Chaohui Chen Wenhui Fang Shugui Lin Hefei Fuzhou Institute of Vegetable Science, Fuzhou, 350111;Purity Identification of Cauliflower ‘Songhua 55 day’ Using SRAP Markers[J];Molecular Plant Breeding;2011-06
3 TAN Jiang-ping1,4,ZENG Xiu-li2,3,LIAO Ming-an1(1.Horticulture College,Sichuan Agricultural University,Ya'an 625014,China;2.Institute of Vegetables,Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850030,China;3.Maize Research Institute,Sichuan Agricultural University,Ya'an 625014,China;4.Shizhu Agriculture Committee,Shizhu 49100,China);Genetic diversity of natural Prunus mira populations detected by SRAP[J];Acta Prataculturae Sinica;2012-06
4 ZHENG Wei1,2(1.College of Pratacultural and Environmental Sciences,Xinjiang Agricultural University,Urumqi 830052,China;2.Xinjiang Key Laboratory of Grassland Resources and Ecology,Urumqi 830052,China);Research progress on utilization and conservation and of Seriphidium transiliense[J];Grassland and Turf;2013-01
5 LIU Ziji,CAO Zhenmu,YANG Yan(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture,Danzhou,Hainan 571737);Research Progress and Comparative Analysis on Purity Identification of Crop Hybrids by Using Molecular Marker Technology[J];Journal of Changjiang Vegetables;2013-12
6 MI Jun-hong 1,2 ,SHANG Xiao-hong 2,3a ,ZHOU Sheng-mao 2,3a* ,CHE Jiang-lü 3 , HUANG Ru-kui 2,3a* ,LIANG Ren-fan 2,3a ,WEN Jun-li 2,3 ,GUO Yuan-yuan 2,3 ,LIANG He 1 ( 1 Agricultural College,Guangxi University,Nanning 530005,China; 2 Guangxi Crops Genetic and Improvement Biotechnology Lab,Nanning 530007,China; 3a Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China);Optimizing and verification of PCR system for SRAP marker screening related to powdery disease resistance in bitter melon (Momordica charantia)[J];Journal of Southern Agriculture;2013-06
7 GUO Juan;FAN Junfeng;LIANG Jun;College of Forestry,Northwest A&F University;The Research Institute of Forest Ecology,Environment and Protection,The Chinese Academy of Forestry;;Genetic Difference Analysis of Populus deltoides Using SRAP and EST-SSR Markers[J];Acta Botanica Boreali-Occidentalia Sinica;2013-09
8 FAN Aili1,2,HUANG Rukui1,2,ZHANG Lugang3,XU Yujian4,ZHU Lin4 1 Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China 2 Guangxi Crop Genetic improvement and Biotechnoloy Laboratory,Nanning,Guangxi 530007,China 3 College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China 4 Vegetable Technology Promotion Station in HeZhou,Hezhou,Guangxi 542800,China;Research Progress on Heat Tolerance in Crucifer Vegetable Crops[J];Chinese Journal of Tropical Crops;2012-01
9 LI Ying-ying;Department of Garden Engineering,Heze University;;Conserved DNA-derived Polymorphism Marker Technique and Its Application[J];Biotechnology;2013-04
10 Jiang Yan 1,2,Liu Jinping 1(1 Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources(Hainan University),Ministry of Education ;College of Agronomy,Hainan University,Haikou 570228;2 Dehong Tropical Agriculture Research Institute,Ruili Yunnan 678600);Establishment and Optimization of Black Pepper SRAP Reaction System[J];Chinese Agricultural Science Bulletin;2012-31
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Lan 1* , HAN Yan-chuang 1* , PENG Yu-lu 1 , TENG Cai-zhu 1 , ZHOU Ming-quan 2** , HU Zhong-li 1, 2** , SONG Yun-chun 1 ,2 (1 Key Lab of the Ministry of Education for Plant Developmental Biology, Wuhan University, Wuhan 430072;2 Lotus center , Wuhan University, Wuhan 430072, China);Application of ISSR Technique to Genetic Research of Lotus Root[J];Amino Acids & Biotic Resources;2004-03
2 WU Zhi-pu,YANG Wen-xiang,LIU Da-qun,ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Biological Control Center of Plant Pathogens and Plant Pests of Hebei Province, Baoding 071001, China);Establishment of SRAP technique system in wheat genome[J];Journal of Agricultural University of Hebei;2005-03
3 Yang Chuanping, Wei Li, Jiang Jing, Liu Guifeng, Zhao Guangyi(Northeast Forestry University, Harbin 150040, P. R. China);Analysis of Genetic Diversity for Nineteen Populations of Pinus sibirica Du Tour with Technique of ISSR[J];Journal of Northeast Forestry University;2005-01
4 WANG Daojie1,2, TIAN Jianhua1,WANG Hao1,HU Xuanping1,LI Dianrong1,GUO Aiguang2(1 Hybrid Rapeseed Research Center of Shaanxi Province, Dali, Shaanxi 715105,China;2 College of Life Science,Northwest SciTech University of Agriculture and Forestry, Yangling, Shaanxi 712100,China);RAPD markers linked to mono-dominant GMS in rapeseed[J];Acta Botanica Boreali-occidentalia Sinica;2003-09
5 WANG Yan-hua~(1,2),HOU Xi-lin~(1,*),XU Ming-yu~1gricultural University,Nanjing 210095,China;2 College of Horticulture, Hebei Agricultural University, Baoding 071001,China);Study on optimization for ISSR reaction system in Brassica campestris ssp.chinensis using orthogonal design[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
6 Zhang Renbing 1 Yi Ke 2 Xu Yong 3,* Shou Senyan 1 Liu Ligong 3 Zhang Haiying 3 Gong Guoyi 3 1Department of Horticulture, Zhejiang University, Hangzhou, 310029 2College of Science,Hunan Agricultural University, Changsha,410128 3National Engineering Research Center for Vegetables, Beijing, 100089 *Corresponding author,xuyongxy@95777 com;Construction of a Molecular Genetic Map for Watermelon Derived from Recombinant Inbred Lines (RILs)[J];Molecular Plant Breeding;2003-04
7 Ren Yu1,2 Wang Deyuan2* Zhang Yindong1 Li Ying2 Wang Hengming21 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 5711012 Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, 510640*Corresponding author, wdy168@tom.com;Optimization of SRAP-PCR in Hot Pepper (Capsicum annuum L)[J];Molecular Plant Breeding;2004-05
8 Xie Yunhai1* Xia De'an1 Jiang Jing1 Lin Ping2 1 Department School of Forest, Northeast Forestry University, Harbin, 150040; 2 Institute of Heilongjiang Forestry Research, Harbin, 150040;Optimization for ISSR-PCR System of Fraxinus mandshurica Rupr.Using Orthogonal Design[J];Molecular Plant Breeding;2005-03
9 WANG Tong qiang 1, TIAN Zhu ping 1, HUANG Ze su 1, WEI Zhong fen 1, SHAO Ming bo 2 (1. Institute of Oil Crops , Guizhou Academy of Agricultural Sciences, Guiyang 550006; 2 . Guizhou Institute of Oil Crops, Sinan 565109 CHINA);Selection and Breeding of Dominant Nucleic Male Sterile Line with Low Erucic and Glucosinolate Content (Qianyou 2 AB) in B.napus[J];GUIZHOU AGRICULTURAL SCIENCES;1999-05
10 HE Qiao, LIANG Guo-lu, XIE Jiang-hui (College of Horticulture and Landscape , Southwest Agricultural University, Beibei, Chongqing 400716 China;Southern Subtropical Crop Research Institute , Chinese Academy of Tropical Agricultural Sciences ,Zhanjiang, Guangdong 524091 China);Optimization of the inter-simple sequence repeat reaction system and its preliminary application in wax apple(Syzygium samarangens)[J];Journal of Fruit Science;2005-02
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