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《Jiangsu Journal of Agricultural Sciences》 2010-05
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Establishment of SSR Fingerprint Map and Genetic Similarity of Varieties(Lines) in Japonica Rice(Oryza sativa L.)

CHENG Bao-shan,WANG Jian,XU Wei-jun,YANG Jia-yin(Huaiyin Institute of Agricultural Sciences of Xuhuai District,Jiangsu Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake,Huaian 223001,China)  
Genetic similarity of 48 varieties(lines) in japonica rice was analyzed by 114 alleles detected from 47 pairs of SSR primers.17 out of 47 pairs of polymorphic SSR primers were selected for their high polymorphism to construct the fingerprinting maps of the 48 varieries in japonica rice.The results showed that all the 48 varieties could be distinguished from each other by the fingerprint map constituted by 17 pairs of SSR primers.The genetic similarity coefficients of the 48 varieties ranged from 0.65 to 0.98.All the varieties were classified into three groups at the level of genetic similarity coefficient of 0.69 according to cluster analysis by an un-weighted pair-group average method with arithmetic mean.Most of the varieties were classified into the third group.
【Fund】: 国家高技术研究发展计划(2010AA101302);; 国家科技支撑计划资助子项目(2006BAD01A01-7);; 江苏省科技成果转化专项基金项目(BA2008076);; 江苏省环洪泽湖生态农业生物技术重点实验室开放基金资助项目(HZHL0820)
【CateGory Index】: S511.220.353
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