Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Jiangsu Agricutural Research》 2001-01
Add to Favorite Get Latest Update

CLONING AND SEQUENCE ANALYSISING OF GLYCOPROTEIN I (gI) GENE OF MAREK′S DISEASE VIRUS FOR DIFFERENT STRAINS

DING Jia bo, ZHAO Wen ming, JI Rong, JIN Wen jie, CUI Zhi zhong ( Dept of Vet Med, Ani Sci and Vet Med Coll, Yangzhou Univ, Yangzhou 225009, China)  
Glycoprotein I (gI) genes were amplified from genomic DNA of MDV 814 and G 2 strains by PCR. The amplified fragments were cloned into pUC18 vector plasmid via restriction endonuclease Bam HⅠ and Sma Ⅰ repectively. The recombinants were screened by restriction endonucleas analysis. The obtained positive gI genes marked 814 gI and G 2 gI were sequenced and compared with the other reported gI sequences of MDV. The homology of gI for different MDV strains was analysised and the phylogentic tree was also drawed.
【Fund】: 国家自然科学基金资助项目 !(3 9870 0 0 8)
【CateGory Index】: S855
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved