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《Jiangsu Agricutural Research》 2001-02
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PREPARATION OF MONOCLONAL ANTIBODIES SPECIFIC FOR MDV GLYCOPROTEIN I EXPRESSED IN ESCHERICHIA COLI

HAN Ling xia 1,CHEN Lei 1,DING Jia bo 1,CUI Zhi zhong 1,2 (1. Dept of Vet Med, Ani Sci and Vet Med Coll, Yangzhou Univ, Yangzhou 225009, China; 2. Ani Sci and Tech Coll, Shandong Agric Univ, Taian 271018, China)  
The fusion product of glycoprotein I of very virulent plus (vv +) MDV 648A strain expressed in Escherichia coli was discerned and excised from SDS PAGE, then inoculated BALB/C mice directly as immunogen to prepare specific monoclonal antibody. 3 strains of monoclonal antibody hydridoma cells were obtained by IFA, and all were specific to MDV infected CEF through dot ELISA. This is the first report on gI Mab that is responsive to MDV in cell cultures through Western blotting. These Mabs might be used to elicit the possible relationships between MDV variation and expression of gI in virus infected cells.
【Fund】: 国家自然科学基金资助项目 (3 9870 0 0 8) ;; 国家高新技术发展计划项目 (863 10 10 5 0 3 0 2 )
【CateGory Index】: S855
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