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《Journal of Yandzhou University》 2006-02
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Ultrafiltrating analysis of plant protein hydrolysates and the effect of hydrolysates on the growth of the mouse embryo fibroblasts

LIU Wen-bin,WANG Tian(Coll of Anim Sci and Tech,Nanjing Agric Univ,Nanjing,210095,China)  
Papain,trypsin and As1.398 subtilisin were used to degrade soybean meal,cottonseed meal,rapeseed meal and peanut meal separately,then used enzymatic degradation products to ultrafiltrate and to study the effect on the cell culture of the mouse embryo fibroblasts.The results indicated that the distribution of the molecular weights of the enzymatic degradation products was related to the kinds of the enzymes and the substrates,and As1.398 subtilisin was the most degraded enzyme for the soybean meal,the percentage of the molecular weight which was less than 3 ku in the enzymatic degradation products was 31.67%;all enzymatic degradation products could promote the growth of the mouse embryo fibroblasts,and the most effective enzymatic degradation products was the cottonseed meal degraded by As1.398 subtilisin,the speed of cell growth of that was higher than that of enzymatic degradation products of the peanut meal,rapeseed meal and soybean meal.
【Fund】: 江苏省“十五”科技攻关项目(PJ2003-39);; 国际合作研究项目(IU2002-43)
【CateGory Index】: TS229
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