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《Journal of Yangzhou University(Agricultural and Life Science Edition)》 2008-02
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Study on the method to investigate the mutation in IGF-2 gene intron 3 G3072A and the distribution of polymorphism in different pig breeds

YU De-bing1,ZHANG Wei-feng2,HE Zong-liang1,WANG Lin-yun1,HUANG Rui-hua1,DU Wen-xing1,LIU Hong-lin1(1.Coll of Anim Sci and Tech,Nanjing Agric Univ,Nanjing 210095,China;2.Coll of Agron,Hebei Univ of Engin,Handan 056038,China)  
A mispairing PCR was used to flank the paternal expressed IGF-2 gene intron 3 G3072A mutation in 314 pigs.The results showed that the mispairing PCR was a rapid,simple and efficient method to detect single nucleotide polymorphism(SNP).The single nucleotide polymorphism(G→A transition) in IGF-2 gene intron 3 3072 site was significantly different between the imported populations and Chinese native populations(P0.01).The A allele frequencies of external breeds were higher than the local breeds.GA and AA genotypes in imported populations(Landrace,Large White and Duroc pigs) reached 60%-70% and 20%-30%,respectively.Sutai pigs from Landrace×Large White×Erhualian cross frequencies of GA genotype had reached 78%,and were the significantly higher than Chinese native populations.
【Fund】: 江苏省高新技术研究计划项目(BG2004317 BG2005304)
【CateGory Index】: S828.2
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