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《江苏农学院学报》 1995-01
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STUDY ON EMBRYO CLONING AND RECLONING IN GOATS

Cheng Yong ;Sheng Zhangmei ;Zhou Xiangang ;Du Miao ;Xu Shaofu(Dept.of Anim. Sci.and Vet.Med.,Jiangsu Agric,Coll.,Yangzhou 225009)  
Blastomeres or cells of various dedifferentiating and developing stages from goats were injectedinto the perivitelline space of enucleared oocytes, the two cells were fused by electrofusion. The cloning eggs(first generation)cultured for 5 days in vivo were used as karyoplast donors to reconst itute recloni ng eggs.Recloning eggs(second generation) can be obtained by salne procedure as cloning eggs, 129 cloning eggs and143 recloning eggs(second generation) were transfered into the oviducts of synthetic ewe's reclpients,2 ofcloning and 4 of recloning offsprings have been born. This paper discussed main points of cloning techniqueand efficiency in the goats.
【Fund】: 国家863高技术研究基金
【CateGory Index】: S827.3
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【References】
Chinese Journal Full-text Database 1 Hits
1 YIN Lie, GENG Yue-bing, LIU Jian, CHENG Yong~* 225009, China);Preliminary Study of Gene Targeting in Goat Fetal Fibroblasts[J];扬州大学学报;2004-01
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 LU Jian hong, PENG Da xin, WU Yan tao, ZHANG Ru kuan, LIU Xiu fan ( Key Lab of Ani Infec Dis, MOA, Yangzhou Univ , Yangzhou, 225009, China);AMPLIFICATION OF FULL-LENGTH HEMAGGLUTININ AND NEURAMINIDASE GENES OF H9N2 AVIAN INFLUENZA VIRUS AND GENE SEQUENCE COMPARISON[J];扬州大学学报;2003-01
2 Cheng Yong ;Sheng Zhangmei ;Zhou Xiangang ;Du Miao ;Xu Shaofu(Dept.of Anim. Sci.and Vet.Med.,Jiangsu Agric,Coll.,Yangzhou 225009);STUDY ON EMBRYO CLONING AND RECLONING IN GOATS[J];江苏农学院学报;1995-01
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