THE USE OF CONDITIONED MEDIUM OF MOUSE MUSCLE AS A COLONY STIMULATING FACTOR FOR THE CULTURE OF MOUSE BONE MARROW MACROPHAGE CELLS
Ge Zhon-liang Liu Xiu-zhen (Institute of Radiation Medicine, Academy ot Military Medical Sciences)
The use of conditioned medium of mouse muscle (CMMM) as a colony stimulating factor (CSF) for in vitro culture of macrophage cells from mouse bone marrow was repoited. The morphology, phagocytosis and digestion of macrophage cells were studied. It was shown that CMMM could stimulate bone marrow cells to produce macrophage cells principally. The macrophage appeared in the medium after culture of culture of mouse bone marrow nucleated cells for 2—3 days, but its phagocytosis and digestion were shown markedly after culture for 6 days. The number of GM-CFU-C after culture of mouse bone marrow cells by CMMM was increesed. It was possible that CMMM could stimulate CFU-S to be changed into GM-CFU-C. The method of macrophage culture from bone marrow cells by CMMM is quite easy to handle and useful for study of generation, development and function of macrophages.