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《Bulletin of the Academy of Military Medical Sciences》 1988-04
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A SIMPLE METHOD FOR DETERMINING IL-2 ACTIVITY USING ACTIVATED SPLEEN CELLS AS RESPONSED CELLS

Zhang Mingwei;Li Yang;Yao Zhijian Institute of Basic Medical Sciences,Academy of Military Medical Sciences  
IL-2 dependent cell line such as CTLL has been widely used for determining IL-2 activity.It has been proved that such cell line provides high specificities to IL-2,but it could be unstable during passage.Here we reported a method in which activated spleen cells were used as responsed cells.Some parameters which effect the determination were investigated,Meanwhile a comparison between our method and the method using a IL-2 dependent cell line,CTLL-2,was also made.The results showet thad CTLL and activated spleen cells provided similar sensitivities to IL-2. We concluded that the method using activated spleen cells for determining IL-2 activity is a very reliable and simpler procedure.
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