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《Bulletin of The Academy of Military Medical Sciences》 2001-04
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Genotyping of Pseudomonas aeruginosa by randomly amplified polymorphic DNA analysis and its application

YE Ming_Liang 1, WU Hai_Huan 1, WANG Quan_Li 2 (1.Jinan Military General Hospital, Jinan 250031, China; 2. Institute of Field Transfusion, Academy of Military Medical Sciences, Beijing 100850, China)  
Objective:To type Pseudomonas aeruginosa (PA) with randomly amplified polymorphic DNA(RAPD) analysis. Methods: Optimal reaction system and amplification condition of RAPD were used for genotyping of the 274 strains PA isolated from the clinical samples and the genotype map was drawn according to. At the same time, the continuity or skipping type of PA infection in 8 individuals was observed and analyzed. Results:From the 274 strains of PA, 133 kinds of PA RAPD type were distinguished by RAPD and the skipping type was observed in 1 individual with PA infection. Conclusions: One hundred and thirty three kinds of PA RAPD type were obtained by RAPD in 274 PA strains and the multi_strain infection with the same species of PA was found in individual infection.
【Fund】: 全军医药卫生“九五”青年基金资助课题 ( 98Q0 12 )
【CateGory Index】: R378
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