Protective effect of Jia-wei Sheng-mai-san on renal ischemia-reperf usion injury in rats
LI Jing ~(1,3), QUE Hai-Ping ~(2), LIN Qiu-Xia ~(2 ), L Sh uang-Hong ~(2), GAO Jun-Yu ~(1*) (1.Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100039,China; 2.Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing, 100850, China; 3.Tongzhou District Hospital, Beijing 101100 ,China)
Objective: To reveal the protective effect of Ji a-wei Sheng-mai-san on renal ischemia-reperfusion injury in rats. Methods: Eighteen rats were divided randomly into three groups: group 1 ,sham operation; group 2, water (vehicle)-treated and group 3, Jia-wei Sheng- mai-san-treated. The rats in group 2 and group 3 were subjected to 45 min of w arm (renal) ischemia and then received reperfusion for 24 h. At the same time, the rats in group 1 received sham operation. Three 3 days before renal ischemia operation, Jia-wei Sheng-mai-san was administrated to rats of group 3.Results:Rats (administrated) with Jia-wei Sheng-mai-san ha d less functional impairment of the kidneys than those in vehicle-treated group . The histological changes were not as severe as those of controls.Compared with vehicle-treated group, increases in serum (creatinine) and blood urea nitrog en 24 h after reperfusion were attenuated by 55.9% (P=0.011) and by 42.8% ( (P=0.024)), respectively in group 3. The ischemia-reperfusion-induced in creases in tissue myeloperoxidase(MPO)and malondialdehyde(MDA) were reduced by 68% (P 0.001) and 59%( P 0.001) respectively in group 3.Tubular ce ll necrosis in medulla was less pronounced (P 0.05) in animals administrate d with Jia-wei Sheng-mai-san. Conclusion: Jia-wei Sheng-m ai-san could partly mitigate renal damage or failure after ischemia-reperfusio n injury of the kidney by inhibition of neutrophil infiltration and oxidation in jury.
【CateGory Index】： R285