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《Bulletin of the Academy of Military Medical Sciences》 2009-02
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Expression and identification of mycobacteriophage D29 lysis gene

YANG Wen-Hui,YU Long,HU Ling-Fei,WEN Zhan-Bo,LI Jin-Song*(National Research Center of Bio-Protection Equipment and Engineering,State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China)  
Objective:To express mycobacteriophage D29 putative lysis gene in Mycobacterium smegmatis and identify its muralytic activity.Methods: Homological sequences of 89 ORFs encoded by mycobacteriophage D29 were blasted against DNA sequences in GenBank for possible functional identification,and ORF gene10 was found to be highly homological to lysins.This gene was cloned into E.coli-Mycobacterium shuttle plasmid pUV15tetORm and electroporated into M.smegmatis mc2155.The expressed product was examined by SDS-PAGE.The lytic activity was identified by culture turbidity measurement and transmission electron microscopy(TEM).Results and Conclusion: A recombinant shuttle plasmid pUV-gene10 was successfully constructed and gene10 was successfully expressed in Mycobacterium.The expressed product can effectively reduce the turbidity of host strain culture by hydrolyzing its cell wall.It is proved that gene 10 is a phage lysin,which is hypothesized to be a non-classical secretion protein.
【Fund】: 国家科技重大专项(2008ZX100/03-016)
【CateGory Index】: R346
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【Co-references】
Chinese Journal Full-text Database 4 Hits
1 WANG Shuang, HAO Li-mei, DUAN Hui-li, et al. National Bio-protection Engineering Center,Institute of Medical Equipment,Academy of Military Medical Sciences,Tianjin 300161,China;Application and Prospect of Bacteriophage Lysins in Medicine[J];Journal of Environment and Health;2009-02
2 ZHU Cheng-Gang1)*, DING Zhi-Shan2) (1)Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310029, China; 2)Department of Life Science, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China);Expression and Function Analysis of Mycobacteriophage D29 Chitinase Gene[J];Progress In Biochemistry and Biophysics;2005-08
3 LI Xiao-Jing 1,2, ZHANG Hao1, FU Xue-Qi2, LI Yan-Ying1, CHEN J ing1, LI Yu-Ling1, FANG Hong-Qing 1* and CHEN Hui-Peng 1* 1.Institute of Biotechnology,Academy of Military Medical Sciences, Beijing 10 0071, China 2.College of Life Science, Jilin University, Changchun 130023,China;Expression and Bioactive Characterization of Bacteriophage Lysin Gene of Bacil lus anthracis in Escherichia coli[J];Chinese Journal of Biotechnology;2005-02
4 WANG Li-zhe,LEI Lian-cheng,HAN Wen-yu (College of Animal Science and Veterinary Medicine ,Jilin University,Changchun 130062,China);Construction of Prokaryotic Coexpression Vector for Lysis Gene E and Staphylococcus Nuclease Gene and Preparation of E. coli Bacterial Ghost[J];Chinese Journal of Biologicals;2007-08
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