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Characteristics of a rat model of diabetic nephropathy induced by streptozotocin

ZHU Xin-Kai1,2,HUANG Lin-Yi1,HUANG Rui1,2,HAN Chun-Guang1,WANG Qiong1,LIU Yong-Xue1 *(1.Beijing Institute of Radiation Medicine,Beijing 100850,China;2.Jiangxi College of Traditional Chinese Medicine,Nanchang 330004,China)  
Objective:To establish a rat model with diabetic nephropathy(DN) and clarify its characteristics.Methods: Sixty Wistar rats were randomly divided into the control and DN model groups of thirty rats each.The DN model of rats was induced by a single dose of intraperitoneal injection of streptozotocin(STZ) at a dose of 65 mg/kg,and the control rats were injected with an equal volume of citric acid buffer.Clinical and pathological parameters of the rats,including blood glucose,body mass,protein and creatinine of 24-hour urine kidney weight and pathological changes were examined or determined at weeks 6,10,12,16 and 32 after injection.Results:Compared with control rats,persistent hyperglycemia,decreasing body mass and increasing 24-hour urine protein and urine protein/urine creatinine ratio were determined with progression of the disease.In the meantime,pathological examination indicated that the kidney hypertrophy of model rats appeared early,while the changes in glomeruli and renal tubules began to appear at the 10th week,and were gradually aggravated to the stage with the typical lesions of DN in kidneys,including thickening of glomerular mesangium,extracellular matrix hyperplasia and renal tubulo-interstitial damages etc.Conclusion: STZ injection could induce a persistent hyperglycemia in rats,which then could result in the key changes of DN beginning at about 12 weeks after injection.
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