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《Bulletin of the Academy of Military Medical Sciences》 2010-06
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Tim-3 in regulating the activity and phenotype of macrophage

GENG Shao-xia1,2,LI Yu-rong2,HOU Chun-mei2,WANG Hui-hui2,ZHANG Ji-lu2,HAN Gen-cheng2,SHEN Bei-fen2,MA Yuan-fang1,LI Yan2(1.Department of Molecular Immunology,Medical College of Henan University,Kaifeng,Henan 475001,China;2.Department of Molecular Immunology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,China)  
Objective To investigate the role of Tim-3 in regulating the activity and phenotype of macrophage.Methods RNA interference(RNAi) technique was used to knock down the Tim-3 expression in macrophage cell line RAW264.7.The role of Tim-3 in regulating the activity of macrophage was investigated by ELISA and flow cytometry respectively.Results Recombinant Tim-3 siRNA vector and control vector were transfected into RAW264.7 cell lines by Lipofectamine 2000.After being selected by G418,the RAW264.7 cell lines stably expressing Tim-3 siRNA were successfully established.ELISA data showed that activation of Tim-3 pathway enhanced TNF-α and IL-6 secretion from macrophage.Interference of Tim-3 expression affected the expression of CD80/CD86 on the surface of macrophage and thus promoted Th1,Th17 polarization.Conclusion Functional assays indicate that Tim-3 plays important roles in promoting TNF-α and IL-6 production by macrophage.More importantly,Tim-3 is involved in the regulation of CD80/CD86 expression on macrophage and promotes Th1 and Th17 polarization.
【Fund】: 国家自然科学基金面上项目(81072475);; 国家重点基础研究发展计划(973计划)项目(2007CB512406)
【CateGory Index】: R392.12
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