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The time-course effect of perfluoroisobutylene inhalation on endogenous nitric oxide and its synthases activity in rats

WANG He-mei,HUANG Chun-qian,ZHANG Xian-cheng,QU Wen-sheng,SUN Xiao-hong, DING Ri-gao,RUAN Jin-xiu(National Beijing Center for Drug Safety Evaluation and Research,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China)  
Objective To investigate preliminarily the role of nitric oxide(NO) in perfluoroisobutylene(PFIB)-induced acute lung injury(ALI).Methods Male SD rats were randomly divided into one control group and six PFIB-exposed groups which were executed at 1 h,2 h,4 h,8 h,16 h and 24 h after PFIB exposure,respectively,with 4 rats in each group.The PFIB-exposed groups were exposed to PFIB at the concentration of 140 mg/m3 for 5 min in a flow-past whole-body chamber while the control group was exposed to the filtered air in a similar manner.After execution at the corresponding time-point,the right lung,serum and brochoalveolar lavage fluid(BALF) from the left lung were collected and the nitric oxide synthase activity(both the endothelial and the inducible types,namely eNOS and iNOS),the content of NO in the serum and BALF were measured.Results During the first 8 hours after PFIB exposure,the activity of eNOS in the lung tissue showed a trend of gradual declination but with no statistical significance detected when compared with the control group.The activity of iNOS in the lung tissue demonstrated an opposite tendency.Eight hours after PFIB exposure both types of NOSs began to gradually return to their baselines.The content of NO in the serum and BALF showed similar change profiles to those of iNOS activity.Conclusion No dramatic changes in the content of NO or in the activity of NOSs were found,suggesting the pathological significance of the endogenous NO system in the PFIB-induced ALI needs to be further clarified.
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