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《Mycosystema》 1994-01
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ISOLATION OF GENOMIC DNAS FROM FUNGI USING BENZYL CHLORIDE

Zhu Heng Qu Feng Zhu Lihuang (Institute of Genetics, Academta Sinica, Beijing 100101)  
With rapid progress of biotechnology, the difficulty to isolate high quality DNAs becomes a main obstacle. Almost all of the reported fungus DNAs extraction protocols include homogenizing in liquid nitrogen or digested with enzymes to destroy cell walls and membrances. Thus, these treatments are time consuming and tedious. Benzyl chloride is a chemical reagent which, under weak alkalic conditon, can react with hydroxyl residues in polysaccharides, such as chitin, thus destroy the sugar chain of fungal cell walls. Genomic DNAs are then released with the least mechanic shearing. Here, with the use of benzyl chloride, a simple, fast and inexpensive procedure for fungal genomic DNA preparation is developed. With this method, the fungal genomic DNAs are extracted with stable yields and little contamination of proteins or other organic components. The isolated DNAs are suitable for digestion with restriction endonucleases, RFLP analysis, PCR amplification and other molecular biological experiments. No report concerning DNA preparation with benzyl chloride has been read ever before in national or international publications.
【Fund】: 863生物领域第一主题资助
【CateGory Index】: Q933
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