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《MYCOSYSTEMA》 2000-01
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STUDY ON RELATIONSHIP AMONG SPECIES OFAMANITA USING RAPD

CHEN Zuo-Hong;ZHANG Zhi-Guang;ZHANG Tian-Xiao(College of Life Science, Hunan Normal Universing, Changsha, 410081)  
Random amplified polymorphic DNA (RAPD) was used in analysing the relationship amongtwenty-six speices of Amanita from Mangshan,Hunan. of 40 primers, 6 ones generated repreducible RAPDprofiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species ofAmanita by its RAPD profiles. The similarity coefficients obtained from profiles genelated by six primers amongAmanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clusteringanalysis. It indicated that there were tWo groups in 26 species and some species with ring and membrmousvolva clustered a group which coincided with morphological classification.
【Fund】: 国家科委重点课题资助!93(7)-03
【CateGory Index】: Q939.5
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