Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《MYCOSYSTEMA》 2000-01
Add to Favorite Get Latest Update

PURIFICATION AND SOME PROPERTIES OF XYLANASEFROM ASPERGILLUS NIGER

CHEN Hong-Ge; ZHU Jing; LIANG Gai-QinYAN Zi-Zheng;JIA Xin-Cheng; ZHANG Shu-Zheng(Institute of Microbiology, Academia Sinica,Beijing 100080)  
Zymograms of the cultrual supernatant of Aspergillus niger 149 showed xylanase's two bandstentatively named X-I and X-II. X-I and X-II were purified to PAGE homogenity by (NH4)2So4 fractionationand DEAE-Sephadex A50 column chlomatography. The molecular mass of X-I and X-II eshmated by SDS-PAGEand concentrahon gradient PAGE were 37kDa,76kDa,24kDa, and 23kDa. respechvely. X-I and X-II contained27.6% and 7.3% carbohydrate residues, respechvely. The optimal reachon conditions for X-I and X-II were at50℃ pH4.6 and 55℃ PHS.2. X-I and X-II retained 100% and 2.8% respechvely, of the original activity afterheating at 50℃ for 24h. The achvihes of X-I and X-II were considerably inhibited by HgCl2 and AgNO3. Thehydrolysis products from different xylan with X-I and X-II were different.
【Fund】: 国家自然科学基金!39600029;;国家“九五”科技攻关项目!No.96-C03-02-03
【CateGory Index】: Q936
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 CHEN Hong-ge1,ZHAO Wei-li1,LI Rui-feng2,JIA Xing-cheng1(1.College of Life Sciences,Henan Agricultural University,Zhengzhou,Henan Province,450002;2.Henan Lianhua Monosodium Glutamate Co.Ltd.,Xiangcheng,Henan Province,466200);Screening of mutant with high yield of both β-glucanase and xylanase and its application in wheat processes[J];Industrial Microbiology;2007-05
2 WANG Miao,WANG Jun-fang,HE Jie,YANG Yuan-yuan,ZHANG Er-fang (College of Life Science,Henan University,Henan Kaifeng 475004,China);Screening,Identification and Preliminary Study of a Wheat Straw-Degrading Filamentous Fungi[J];Journal of Henan University(Natural Science);2012-02
3 QIAO Yu MAO Ai-Jun HE Yong-Zhi LIU Wei-Feng DONG Zhi-Yang (Institute of Microbiology, Academia Sinica, Beijing 100080, China);SECRETED EXPRESSION OF TRICHODERMA REESEI ENDO-β-GLUCANASE Ⅱ GENE IN PICHIA PASTORIS AND ANYLYSIS OF ENZYMIC PROPERTIES[J];;2004-03
4 LI Hai-Yan MAO Ai-Jun HE Yong-Zhi DONG Zhi-Yang* (Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China);CO-EXPRESSION OF GLAA AND XYNB OF ASPERGILLUS NIGER IN INDUSTRIAL YEAST[J];;2004-04
5 CHEN Wei-Wei JIANG Zheng-Qiang* WANG Rui-Jun College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Purification and characterization of an extracellular xylanase from Thermomyces lanuginosus[J];Mycosystema;2009-04
6 MAO Lianshan1,2,SONG Xiangyang1,YONG Qiang1,YANG Fuguo3,YAO Chuncai1,YU Shiyuan1(1.Nanjing Forestry University,Nanjing 210037,China;2.South China University of Technology,Guangzhou 510541,China;3.Central South University,Changsha 410083,China);Purification and Characterization of Xylanases from Trichoderma reesei[J];Journal of Nanjing Forestry Unversity;2002-06
7 LIU Xin-yu~1,CHEN Hong-ge~1, LIU Qing-jun~2, ZHANG Shi-min~1, JIA Xin-cheng~1 (1. College of Biotechnology and Food Science, Henan Agricultural University, Zhengzhou 450002, China; 2. Henan Lotus Gourmet Powder Group Co. Ltd.,Xiangcheng 466200, China);Comparative Study on Enzymic Properties of Xylanases by the Mutant and Wild Strain of Aspergillus niger[J];Journal of Henan Agricultural University;2005-01
8 Ding Changhe, Jiang Zhengqiang, Li Lite, Li Xiuting, Zhang Yan (College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China);Purification of XynB from Streptomyces cirratus[J];Journal of China Agricultural University;2003-06
9 BAO Yi-Hong LIU Wei-Feng MAO Ai-Jun DONG Zhi-Yang** (Institute of Microbiology, Chinese Academy of Science, Beijing 100080, China);Selection of an Alkali-xylanase Production Strain and Its Enzyme Production Condition and Application to Biobleaching of Wheat Straw Pulps[J];Journal of Agricultural Biotechnology;2005-02
10 Cao Xianglin,Wang Junli,Nie Guoxing,etal;Studies on the stability of xylanase produced by Trichoderma koningii[J];China Feed;2009-16
【Citations】
Chinese Journal Full-text Database 2 Hits
1 JIANG Jun-Ping; YAN Zi-Zheng and ZHANG Shu-Zheng(Institute of Microbilogy, Academia Sinica, Beijing100080);The Mode of Action of Xylanase from Aspergillus phoenicis on Xylooligosaccharides[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1995-03
2 Chen Hongge Zhu Jing Liang Gaiqin Yan Zizheng Zhang Shuzheng (Institute of Microbiology, Chinese Academy of Sciences, Beijing 10008 0);SCREENING OF ACIDIC XYLANASE PRODUCING STRAIN AND STUDIES ON ITS ENZYME PRODUCTION CONDITIONS [J];ACTA MICROBIOLOGICA SINICA;1999-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Zhongxing(Department of Chemistry,Ankang University,Ankang 725000,Shanxi);Study on the Optimum Conditions for Xylanase[J];Anhui Agricultural Science Bulletin;2008-09
2 WANG Zhongxing(Department of Chemistry-Life Science,Ankang University,Ankang725000,Shaanxi,China);Study on the Characteristics of Xylanase Produced by Aspergillus oryzae[J];Journal of Ankang University;2008-04
3 XU Juan-juan1,2,GE Chun-mei2,SUN Qin-ying2,ZHANG-Jie2,FAN Mei-zhen1,PAN ren-rui2 (1.Anhui Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei 230036,China;2.Department of Biology & Environment Engineering,Hefei 230022,China);Effects of combinations of microorganisms on lactic acid production using fermentation straw[J];Acta Prataculturae Sinica;2010-06
4 Shi Bo et al (Feed Research Institute of Chinese Academy of Agriculture Sciences,Beijing 100081);Study on the Preparation of Functional Additive Xylooligosacchrides[J];Animal Science Abroad;2000-06
5 Cai Jingmin, Wu Ke, Zhang Jie, He Xiaogang, Pang Renrui (Hefei Union University, Hefei 230022);Production of Xylanase by Aspergillus niger A3 Using Solid State Fermentation[J];Industrial Microbiology;1997-02
6 WANG Shi-ying~1,LEI Bai-shi~1,YUAN Hong-shui~1,LI Shu-na~2,ZHU Bao-cheng~(1,2) (1.College of Life Science,Agricultural University of Hebei,Baoding 071001,China;2.College of Life Sciences,Hebei University,Baoding 071002,China);Mutagenesis Breeding of the Feed Enzyme-complex Producing Strain[J];Journal of Hebei University(Natural Science Edition);2006-01
7 FAN Mei-Xia1,SUN Xun2,TAO Zong-ya1(1.College of Life and Science,Sichuan Normal University,Chengdu 610068,China;2.Department of Life and Science,Heze University,Heze 274015,China);Research Advances on Xylanases[J];Journal of Heze University;2009-02
8 FAN Mei-Xia1,2 FU Can1 SUN Xun1 TAO Zong-Ya2 1Department of Life Science,Heze University,Heze 274015,China 2College of Life Science,Sichuan Normal University,Chengdu 610068,China;Purification and characterization of xylanases from white-rot fungus Trametes gallica[J];Mycosystema;2010-01
9 LI Cai xia 1,?FANG Gui gan 1,?LIU Shu chai 2 (1.Institute of Chemical Industry of Forest Products,CAF, Nanjing 210042,?China;? 2.Department of Light Chemical Industry, Northwest Institute of Light Industry,Xianyang 712081,China);METHODS FOR XYLANASE ACTIVITY DETERMINATION[J];Journal of Chemical Industry of Forest Products(Bimonthly);2001-01
10 ;Probing into the Optimum conditions for Measuring the Activity of Xylanase by means of Orthogonal Experiments[J];Cereal & Feed Industry;2003-05
China Proceedings of conference Full-text Database 1 Hits
1 Francisco Puchal, Josep Mascarell (Universitat Autonoma de Barcelona Spain; Industrial Tecnica Pecuaria S. A. Spain);Feed Enzymes in Poultry and Swine Nutrition[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 JING Mei-zhen et al(College of Life Science,Henan Agricultural University,Henan,Zhengzhou 450002);Screening for High-yield Strain of Xylanase Produced by Engineered Pichia pastoris and Optimization of the Methanol Induced Conditions[J];Journal of Anhui Agricultural Sciences;2008-29
2 WU Xiao-qin et al (Neijiang Normal University,Key Laboratory for Research and Utilization on Regional Special Agricultural Resources of Colleges and Universities in Sichuan Province,Neijiang,Sichuan 641112);Isolation of Cellulase-producing Bacteria and Determination on Their Enzyme Activities[J];Journal of Anhui Agricultural Sciences;2009-35
3 ZHOU Chen-yan et al(Department of Life Science and Technology,The Key Laboratory of Medical Genetics and Molecular Targeting Drugs,Henan Province,Xinxiang Medical University,Xinxiang,Henan 453003);Optimization of Culture Conditions for Xylanase Production by the Solid-state Fementation with Aspergillus niger[J];Journal of Anhui Agricultural Sciences;2010-21
4 Xie Xiangming; He Xiaoqing. School of Biological Science and Biotechnology, Beijing For. Univ.,100083,P. R. China.;Research development of microbial xylanases and their use for bio-bleaching[J];Journal of Beijing Forestry University;2003-03
5 Zhang Jie; Huang Zhenya. School of Material Science and Technology, Beijing For. Univ., 100083, P. R. China.;The influence of substrate ingredient on the synthesis of xylanase from Trichoderma[J];Journal of Beijing Forestry University;2003-06
6 LIU Beidong;YANG Qian;ZHOU Qi;SONG Jinzhu;CHEN Dianfu;LIU Heng;ZHAO Min.Department of Biotechnology, Harbin Institute of Technology, 150001,P.R.China.;Cloning and expression of the endo-β-glucanase Ⅰ cDNA gene from Trichoderma viride AS3.3711.[J];Journal of Beijing Forestry University;2004-06
7 DONG Qing-hua,SHEN Yuan-yue*(Depertment of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China);Research Progress and Prospects on Yeast Expression Systems[J];Journal of Beijing University of Agriculture;2008-02
8 ZHAN JiXun,LIU TingZhi,TIAN ShengYan,WANG DePei,DING YouFang(School of Food Science and bioengineering,Tianjin University of Science and Technology, Tianjin 300222, China);STUDIES ON PROPERTIES AND APPLICATIONS OF XYLANASE PRODUCED BY ASPERGILLUS NIGER S13[J];Journal of Beijing Technology and Business University (Natural Science Edition);2003-02
9 Hong Jiong; Huang AXiuli (Department of Biology, Beijing Normal University 100875,Beijing, China);PURIFICATION AND PROPERTIES OF CELLULASE FROM ASPERGILLUS NIGER VAR[J];JOURNAL OF BEIJING NORMAL UNIVERSITY(NATURAL SCIENCE);1998-03
10 HUANG Yun-hong 1, LONG Zhong-er 1, LI Su-zhen 1, PEI Li-li 2, WANG Shui-xing 2, YU Min 2 (1.Institute of Chemistry and Biological Sciences, Jiangxi Normal University, Nanchang 330027,China;2.Jiangxi-OAI Joint Research Institute,Nanchang 330047;Isolation and Identification of Prolific Microorganism in Xylanase[J];Journal of Jiangxi Normal University (Natural Sciences Edition);2002-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YANG Shu-yan et al(School of Food Science,Southern Yangtze University,Wuxi,Jiangsu 214036);Study on the Preparation of Xylooligosacchrides from Corncob with Acid-xylanase Hydrolysis[J];Journal of Anhui Agricultural Sciences;2007-12
2 ZHANG Hui et al (School of Life Science,Liaocheng University,Liaocheng,Shandong 252059);Recent Research Advance in the Biodegradation of Cellulose[J];Journal of Anhui Agricultural Sciences;2007-27
3 FU Dan-dan et al (College of Food and Biological Engineering,Henan University of Science and Technology,Luoyang,Henan 471003);Studies on Purification and Properties of Xylanase from Fermentation Broth[J];Journal of Anhui Agricultural Sciences;2008-28
4 XUE Ying-li et al(College of Food,Sichuan Agricultural University,Yaan,Sichuan 625014);Selection of High Xylanase Yielding Monascus pupureus Bacterium Strain and Its Properties of Enzyme[J];Journal of Anhui Agricultural Sciences;2011-20
5 FU Guan-hua et al(Department of Life Science and Technology,The key Laboratory of Medical Genetics and Molecular Targetingdrugs,Xinxiang Medical College,Xinxiang,Henan 453003);Research Progress of Xylanase and Its Applications[J];Journal of Anhui Agricultural Sciences;2011-35
6 YANG Li-na et al(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100);Isolation and Identification of a Strain Producing Thermostable Cellulase and Its Enzymatic Characteristics[J];Journal of Anhui Agricultural Sciences;2012-14
7 Wang Zaigui,Wan Sailuo,Li Xunli(Anhui Agricultural University,College of life Science,Hefei 230031);Study of enzymic features of xylan enzyme of Trichoderma[J];Forest By-Product and Speciality in China;2007-01
8 CHE Keshu,WANG Xiangjing,XIANG Zhidan(College of Life Sciences,Northeast Agricultural University,Harbin 150030,China);Screening of spinosads-producing strain with high yield[J];Journal of Northeast Agricultural University;2008-08
9 HAN Jianchun1,LI Mingyuan1,LI Jie2(1.Food College,Northeast Agricultural University,Harbin 150030,China;2.College of Life Sciences,Northeast Agricultural University,Harbin 150030,China);Dairy-derived anti-hypertension seven peptide tandem gene synthesis and expression in Pichia pastoris[J];Journal of Northeast Agricultural University;2011-08
10 Bao Yihong, Li Xuelong, Yang Chuanping(School of Forestry, Northeast Forestry University, Harbin 150040, P. R. China);Screening Experiment of Xylanase-producing Paenibacillus sp. and Optimization of Production Culture Conditions[J];Journal of Northeast Forestry University;2008-09
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 JIANG Jun-Ping;YAN Zi-Zheng and ZHANG Shu-Zheng(Institute of Microbiology, Academia Sinica,Beijing 100080);Purification and Partial Amino Acid Sequence of Xylanase from Aspergillus phoenicis[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1995-02
2 Liu Yueying Zheng Zhonghui Fu Yuxiao Yang Jinghui(Department of Biology, Xiamen University, Furjian Xiamen 361005);STUDIES ON XYLANASE PRODUCTION BY ASPERGILLUS CLAVATUS 22[J];Microbiology;1993-06
3 Zeng Yucheng Zhang Shuzheng(Institute of Microbiology, Academia Sinica, Beijing);PURIFICATION AND PROPERTIES OF XYLANASES FROM ASPERGILLUS PHOENICIS[J];Acta Microbiologica Sinica;1987-04
4 Zeng Yucheng Zhang Shuzheng(Institute of Microbiology, Academia Sinica, Beijing);SUBSTRATE SPECIFICITIES OF LICHENASE AND XYLANASES FROM ASPERGILLUS PHOENICIS[J];Acta Microbiologica Sinica;1987-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved