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CHEN Hong-Ge; ZHU Jing; LIANG Gai-QinYAN Zi-Zheng;JIA Xin-Cheng; ZHANG Shu-Zheng(Institute of Microbiology, Academia Sinica,Beijing 100080)  
Zymograms of the cultrual supernatant of Aspergillus niger 149 showed xylanase's two bandstentatively named X-I and X-II. X-I and X-II were purified to PAGE homogenity by (NH4)2So4 fractionationand DEAE-Sephadex A50 column chlomatography. The molecular mass of X-I and X-II eshmated by SDS-PAGEand concentrahon gradient PAGE were 37kDa,76kDa,24kDa, and 23kDa. respechvely. X-I and X-II contained27.6% and 7.3% carbohydrate residues, respechvely. The optimal reachon conditions for X-I and X-II were at50℃ pH4.6 and 55℃ PHS.2. X-I and X-II retained 100% and 2.8% respechvely, of the original activity afterheating at 50℃ for 24h. The achvihes of X-I and X-II were considerably inhibited by HgCl2 and AgNO3. Thehydrolysis products from different xylan with X-I and X-II were different.
【Fund】: 国家自然科学基金!39600029;;国家“九五”科技攻关项目!No.96-C03-02-03
【CateGory Index】: Q936
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