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《Mycosystema》 2001-02
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APPLICATION OF SEQUENCING OF 5.8S rDNA, ITS1 AND ITS2 ON IDENTIFICATION AND CLASSIFICATION OF ALTERNARIA AT SEPECIES LEVEL

Wang Hong-Kai Zhang Tian-Yu** Zhang Meng (Systematic Mycology and Lichenology laboratory, Chinese Academy of Sciences. Beijing 100080;Department of Plant Pathology, Shandong Agricultural University, Shandong 271018; Biotechnology Institute, Agricult  
: The sequences coding for the 5.8S rDNA and the flanking internal transcribed spacers (ITS1 and ITS2) were amplified by PCR and sequenced from 9 small-spored species and other 3 large-species in Alternaria. Phylogenetic analysis clearly separated the 2 large-spored species from each other and from 9 small-spored species tested, but small-spore species cannot be clearly separated each other. This indicate that small-spored species tested belong to a natural species group. Traditional taxonomic morphologically distinguished species A.leucanthemi may not belong to Alternaria according to sequence analysis, its taxonomic position require further study.
【Fund】: 国家自然科学基金资助项目!(项目号:39770003)&&
【CateGory Index】: Q949.32
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