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《Mycosystema》 2001-02
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CLONE AND SEQUENCE ANALYSIS OF THE CELLOBIOHYDROLASE II GENE (CBH2) FROM TRICHODERMA KNINGII K801

Zhu Ling-Xiang Yu Wei Liang Gai-Qin Dong Zhi-Yang (Institute of microbiology, Chinese Academy of Sciences, Beijing 100080)  
The cellobiohydrolase II gene, cbh2 was amplified by polymerase chain reaction(PCR) from an enhanced cellulase-producing strain, Trichoderma k?ningii K, and then was cloned to T-easy vector. Sequence comparisons show that cbh2 of T. k■ningii is identical to that of T. reesei with the exception of 2 bp in the coding region, which cause an amino acid substituted in the mature CBHII protein. The similarity values between CBHII gene of T. k?ningii and reported cbh2 gene of Trichoderma reesei is 99.89%. The cbh2 gene of T. k?ningii is 1611bp long, and the coding region is interrupted by three short introns. The derived CBHII protein sequence is 471 amino acids long and three putative N-glycosylation sites is found.
【Fund】: 国家自然科学基金资助项目!(No. 39570403)
【CateGory Index】: Q81
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