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《Mycosystema》 2001-04
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PROTOPLAST PREPARATION FORM DIASPORANGIUM SP.

DONG Hong-Ping YUAN Sheng XU Xu-Shi XIN Ding-Kun (Faculty of Life Science, Nanjing Normal University, Jiangsu 210097)  
The effects of factors, such as lytic enzyme concentration, culture age , osmotic stabilizers, digesting temperature and time, on the production of protoplasts from Diasporangium sp. were investigated and optimum conditions for preparation of protoplasts were obtained. Maximum yield could be obtained with 30-hour-old mycelia by using 4%cellulase and 0.5% snailase stabilized with o.6mol/L mannitol and digesting for 1.0h at 35℃.The regeneration was tested on the solid media containing osmotic stabilizer and about 23.8% of protoplasts regenerated on it.
【Fund】: 江苏省青年基金项目(QB96025)资助。
【CateGory Index】: Q813
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【References】
Chinese Journal Full-text Database 5 Hits
1 Gong Shu-li, Deng Fang-ming, Chen Li-li (College of Food science and technology Hunan Agriculture University, Changsha 410128,China );Preparation of Protoplast of Flammulina Velutipes[J];Modern Food Science and Technology;2007-04
2 Duan Guifang Huang Shiwen** Yan Qiusheng Yu Liuqing(China National Rice Research Institute, Hangzhou 310006, China);Preparation and Regenaration of Drechslera monoceras Protoplast[J];Journal of Agricultural Biotechnology;2003-03
3 ZHAO Mo,DAI Chuan-chao,WANG An-qi,TAO Jie,YONG Ying-hui (Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, China);Study on Protoplast Mutagenagsis of Strains Contain High Contest of Arachidonic Acid[J];Food Science;2008-02
4 CHEN Li-li,MA Mei-hu,ZHOU Chuan-yun,GAO Bi-da(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China);Effects of Different Factors on the Protoplast Preparation and Reproduction in Flammulina Velutipes[J];Biotechnology;2005-05
5 DAI Chuan-chao,YUAN Zhi-lin,WANG An-qi (Biology Science College of Nanjing Normal University, 210097 Nanjing,China);Production of arachidonic acid and eicosapentaenoic acid with organic wastewater of soybean products[J];China Oils and Fats;2004-05
【Citations】
Chinese Journal Full-text Database 7 Hits
1 XING LAI-JUN ZHANG JUN SUN GUANG ZHOU JU-YAN ZHOU YU-LIANG(Department of Biology,Nankai University,Tianjin);THE STUDY OF EXPERIMENTAL CONDITIONS FOR FORMATION AND REGENERATION OF PROTOPLASTS IN TWO ASPERGILLUS ORYZAE AUXOTROPHS[J];;1987-04
2 Li Mingchun; Xing Laijun (Department of Microbiology,Nankai University,Tianjin300071);PROTOPLAST FORMATION AND REGENERATION OF γ-LINOLENIC ACID PRODUCING STRAINS OF MORTIERELLA ISABELLINA[J];MYCOSYSTEMA;1997-01
3 Cao Wenqin; Guo Shunxing; Xu Jingtang;Zhang Jihui; Cheng Xiaomei(Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medicalcollege, Beijing 100094);PREPARATION,REGENERATION AND FUSION OF THE PROTOPLAST OF GANODERMA LUCIDUM[J];MYCOSYSTEMA;1998-01
4 LI Gang;LI Baojian (Biotechnology Research Center, Zhongshan University, Guangzhou 510275);STUDY ON THE SEPARATION AND REGENERATION OF GANODERMA PROTOPLAST[J];MYCOSYSTEMA;1999-01
5 LIU Ji-Hua;YUAN Sheng;DAI Chuan-Chao(Department of Biology, Nanjing Normal University Nanjing 210097);SCREENING AND IDENTIFICATION OF FUNGI CONTAINING ARACHIDONIC ACID AND EICOSAPENTAENOIC ACID[J];MYCOSYSTEMA;2000-03
6 Han Li; Chen Shiping Suo jihong(Chinese PLA General Hospital. Beijing, 100853);STUDY ON EXPERIMENTAL CONDITIONS FOR FORMATION OF PROTOPLASTS IN THE PATHOGENIC RHIZOMUCOR PUSILLUS FIRST-FOUND IN CHINA[J];MICROBIOLOGY;1998-01
7 Liang Ping-yan Liu Hong-di (Institute of Microbiology, Academia Sinica) Xiao Xin-fa (Institute of Antibiotics, North China Pharmaceutical Works);SOME FACTORS AFFECTING THE FORMATION AND REGENERATION OF PROTOPLASTS IN TWO PENICILLIUM CHRYSOGENUM AUXOTROPHS[J];;1981-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Rui,MA Shou-neng,LIU Qun,WU Ke,LIU Bin,PAN Ren-rui (The Chemistry and Biotechnology Department of Hefei Union University, Hefei 230022,China);Studies on the preparation and fusion of protoplast from Lentinula edodes hyphae[J];Journal of Anhui University(Natural Sciences);2001-03
2 LIN Ying et al(College of Life Science,Shenyang Agricultural University,Shenyang,Liaoning 110161);Study on Protoplast Formation Condition of Trichoderma[J];Journal of Anhui Agricultural Sciences;2005-12
3 WANG Qian et al(College of Life Science,Hebei University,Baoding,Hebei 071002);Study on the Thermotolerance of Pleurotus ostreatus Cell Engineering Strains[J];Journal of Anhui Agricultural Sciences;2007-05
4 Cao Junwei Gao Chundong Liu Zhijian (School of Life Sciences, Wuhan Universite, Wuhan 430072);High-Producers of Aminoacylase selected from mutants of Aspergillus oryzae[J];AMINO ACIDS & BIOTIC RESOURCES;1999-03
5 PU Shun-chang,LI Chun-ru,WANG Xiao-yan,HUANG Bo,FAN Mei-Zhen,LI Zeng-zhi(Provincial Key Laboratory of Microbial Pest Control,Anhui Agricultural University,Hefei 230036);Preparation of protoplast from Paecilomyces tenuipes,the anamorph of Cordyceps takaomontana[J];Journal of Anhui Agricultural University;2008-03
6 MA Ji-fang,FAN Yong-shan,ZHANG Jing-bai,DONG Jin-gao (Mycotoxin Laboratory, Agricultural University of Hebei, Baod ing 071001, China);Isolation of protoplasts from Exserohilum turcicum[J];Journal of Agricultural University of Hebei;2003-03
7 Shi Liangen Ke Lintian(Department of Sericultural Science,Zhejiang Agriculture University, Hangzhou 310029,China)Fei Jianming(Sericultural Consulting Center of Huzhou City,Huzhou 313000,China);Studies on the Regeneration and Reversion of Protoplasts From Beauveria bassiana, a Pathogenic Fungus to Bombyx mori L.[J];ACTA SERICOLOGICA SINICA;1999-01
8 HUANG Wen-shu,SHI Qiao-qin,Wu Song-gang ( Bioengineering College,F ujian Teachers University,Fuzhou 3 50 0 0 7,China);Protoplast Formation and Regeneration of the Alkaline Lipase Producing Strain Penicillium expansum K40[J];JOURNAL OF FUJIAN TEACHERS UNIVERSITY(NATURAL SCIENCE);2000-03
9 Qiang Hua 1, Chen Wenlie 2, Zhong Xiurong 2, et al (1.Departement of Microbiology,\ 2.Laboratory of Electron Microscopy, Fujian Medical University, Fuzhou, 350004);Observations on the Formation and Release of the Protoplasts from Streptomyces hygroscopicus FC904[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;1999-01
10 Qiang Hua 1, Huang Jie 2 (1. Department of Microbiology, Fujian Medical University, Fuzhou, 350004 (2. Fujian Institute of Microbiology, Fuzhou, 350007);Factors Affecting the Formation of Protoplasts from Streptomyces hygroscopicus FC904[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;1999-02
China Proceedings of conference Full-text Database 1 Hits
1 WANG Kai-sheng(Department of Technology,Shihezi University,Shihezi,Xinjiang 832003,China);Study Advance on Biological Control of Disease of Gastro-intestinal Nematodes by Nematode-trapping Fungus[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 BAO Li ying 1, GU Jun ling 1, CHE Bao quan 2 (1 School of Chemical Engineering and Materials Science, Beijing Institute of Technology, Beijing100081, China; 2 Beijing Institute for Drug Control, Beijing100035, China);Quantitative Analysis of Four Antibiotics by High Performance Capillary Electrophoresis[J];Journal of Beijing Institute of Technology;2001-02
2 Hong Jiong; Huang AXiuli (Department of Biology, Beijing Normal University 100875,Beijing, China);PURIFICATION AND PROPERTIES OF CELLULASE FROM ASPERGILLUS NIGER VAR[J];JOURNAL OF BEIJING NORMAL UNIVERSITY(NATURAL SCIENCE);1998-03
3 Wu Jinnan Wu Youliang (Changshu College Changshu 215500);Studies on Liquid Culture of Flammulina Velu tipes Mycelium[J];JOURNAL OF CHANGSHU COLLEGE;1999-02
4 Jiang Yue; Chen Feng ;Jiang Qian; Li Fubing; Liang Shizhong(Department of Bioengineering, SCUT, Guangzhou 510641; Department of Botany, UHK, Hongkong; No. 2 Hospital of Baoding, Baeding 071051);Production of ω-3 Polyunsaturated Fatty Acids by High Cell Density Culture of Microalgae[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1998-03
5 MA Ji-fang,FAN Yong-shan,ZHANG Jing-bai,DONG Jin-gao (Mycotoxin Laboratory, Agricultural University of Hebei, Baod ing 071001, China);Isolation of protoplasts from Exserohilum turcicum[J];Journal of Agricultural University of Hebei;2003-03
6 Fan Muzhen; Dong Jingao; Yang Junguo;Shen Shuxing; Yang Wenxiang; Liu Guosheng(Department of Plant Protection, Hebei Agricultural University, Baoding 071001 );Factors Affecting Hyphae Growth and Toxin Production of Alternaria brassicae[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1995-04
7 Wang Jiangzhu; Dong Jingao; Wang Yuzhen(Dept. of pl. protec; Agric. Univ. of Hebei; Baoding 071001 );Status on Pathogenic Mechanism of Non-host Specific Toxins to Plants[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1995-04
8 TANG Mei-jun, YIN Kun-shan, CHEN Xue-fen (Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China);Studies on The Cultural Characteristics and Host Range of The Entomogenous Fungus Paedlomyces Aleurocanthm[J];Journal of Tea Science;2003-S1
9 ZHAO Yun-feng~1,2, LIN He-tong~1,3, LIN Jiao-fen~1, CHEN Shao-jun~1, XI Yu-fang~3 (1.College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;2.College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;3.Department of Food Science and Nutrition, Zhejiang University, Hangzhou, Zhejiang 310029, China);Changes of respiration rate, cell membrane permeability and quality in post-harvest longan fruit[J];;2005-02
10 ZHANG Ju-nian~(1,2),LIN He-tong~(1,3),XIE Lian-hui~3,LIN Qi-ying~3(1.College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;2.Institute of Rice,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350019,China;3.College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Biological characteristics of Lasiodiplodia theobromae Pat.causing black-rotten disease of longan fruit[J];;2005-04
China Proceedings of conference Full-text Database 1 Hits
1 XIE Bao-Gui JIANG Yu-Ji, (College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002,);GENETIC AND BREEDING RESEARCH PROGRESS OF FLAMMULINA VELUTIPES[A];[C];2003
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 HUANG Shi-wen 1,2 YU Liu-qing2 DUAN Gui-fang2 ZHAO Hang2 LUO Kuan1(1.Hunan Agricultural University, Changsha,Hunan, 410128; 2.China National Rice Research Institute, Hangzhou, Zhejiang, 310006);IMPROVEMENT OF BARNYARDGRASS POTENTIAL BIOCONTROL FUNGUS BY NTG INDUCING AND~(60)Co γ-RAY IRRADIATION[J];Acta Agriculturae Nucleatae Sinica;2004-06
2 YAN Hong,YANG Qian(Department of Life Science and Engineering,Harbin Institute of Technology University,Harbin 150001,China);Study on Induction Mechanism of Cellulase Production by Two Bacilli[J];Chemistry and Industry of Forest Products;2006-03
3 SONG Ai-Huan LI Hong-Ye** LIU Xiao-Hong( College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China);Protoplast Isolation and Regeneration in Penicillium digitatum[J];Journal of Agricultural Biotechnology;2004-02
4 GUO Cheng-jin,ZHAO Run (College of Chemistry and Life Science, Tianjin Normal University, Tianjin 300387, China);Study on Protoplast Preparation and Regeneration of Cordyceps sinensis[J];Food Science;2009-05
5 XIE Yu-hong,SONG Wen-hua,GE Yan-hui,JIA Ji-hong,QIANG Yan-yan,CAO Yang,FENG Xin~*(The Environmental and Safety School of Tianjin Technology Universty,Tianjin 300191,China);Purification of Cellulase in Culture Medium[J];Biotechnology;2006-06
6 YANG Feng-ling,JIA Le,REN Xuan(College of Life Science,Shandong Agriculture University,Tai'an 271018,China);Protoplast Preparation and Regeneration of Pleurotus cornucopiae[J];Biotechnology;2007-06
7 GUAN Xian-yue1,DAI Chuan-chao1,*,XU Yu-fen1,ZHU Ming2,ZHANG Kai1 (1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China;2. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China);Hypolipidemic and Antioxidation Effects of Selenium-enriched Mycelia of Diasporangium sp. or/and Milk Vinegar on Mice[J];Food Science;2009-17
8 ZHANG Lei, WU Xing-quan (Department of Biological Engineering, Zhengzhou Institute of Technology, Zhengzhou 450052, China);The Clone and Expression of Cellulase Gene of Trichoderma spp.[J];Journal of Cellulose Science and Technology;2004-04
9 CUI Ying-jin,SUN Yu-mei,YANG Hong,HUA Yan-yan(College of Biology & Food Technology,Dalian Institute of Light Industry,Dalian 116034,China);Research on astaxanthin fermentation by Phaffia rhodozyma with effluent during squeezing process of soybean product[J];China Brewing;2006-02
10 ZHAO Hang,ZHANG Jian-ping,YU Liu-qing(State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China);Protoplasts Fusion Between Exserohilum monoceras and Curvularia lunata with Biparental Inactivate Technique[J];Chinese Journal of Biological Control;2006-03
China Proceedings of conference Full-text Database 2 Hits
1 ZHANG Jian-ping ZHANG Zheng-bo ZHAO Hang YU Liu-qing (State Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China);Protoplast fusion between Exserohilum monoceras and Curvularia lunata and their fusants gene identification[A];[C];2005
2 WANG Hao-ran TIAN Yun-long JIANG Xi-liang GUO Ping ZHU Chang-xiong (Institute of Environment and Sustainaljle Development in Agriculture, The Chinese Academy of Agriculture Sciences, Beijing 100081);Progresses on Trichoderma spp. Molecule Biology Study[A];[C];2005
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHAO JI-DING;ZHANG XIAO-QING Institute of Microbiology,Academia Sinica,Beijing 100080;RESOURCES AND DISTRIBUTION OF GANODERMATACEAE IN CHINA[J];Mycosystema;1992-01
2 Xu Tianyu(Teaching and Research section.Beijing Institute of Business Food,Beijing,100037 );Producting Eicosapentaenoic Acid and Docosahexenoic Acid by Biotechnology[J];FOOD AND FERMENTATION INDUSTRIES;1995-01
3 Zhang Jun Xing Laijun Wang Hongmei(Bioiogy Department of Nan Kai University, Tian Jin 300071);BREEDING OF A HIGH-γ-LINOLENIC ACID YIELD PRODUCING STRAIN AND THE EXTRACTION OF FERMENTATION PRODUCT[J];Microbiology;1993-03
4 Zhang Changkai et al.(Depart of Microbiology Shandong Universivy, Jinan, 250100);STUDY ON REGERATIONAL CONDITIONS OF MONOKARYOTIC MYCELIUM 9101(12) OF LENTINUS EDODE[J];JOURNAL OF MICROBIOLOGY;1994-01
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