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《Mycosystema》 2003-03
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PROTOPLAST ISOLATION AND REGENERATION OF MORCHELLA

CUI Zong-Qiang LUO Xin-Chang (Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, 430070; Key Laboratory of Fermentation,Yunnan University,Kunming,650091)  
For the protoplast isolation of Morchella, the optimal age of mycelia was 3d and 0.6ml/L sucrose was the best osmotic stabilizer. The optimal enzymolysis temperature was 30℃and optimal enzymolysis time was 3h. The highest protoplast yield was 3.35×106/100mg. When 0.6mol/L sucrose was used for stabilizer of the regeneration medium of CYM, the regeneration frequency of protoplast was 0.171%. These results and data would provide important parameters for the strain improvement of Morchella through the protoplast technique.
【Fund】: 云南大学发酵重点实验室资助项目
【CateGory Index】: S646.701
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