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《菌物学报》 2004-04
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CULTIVATION AND POLYSACCHARIDE EXTRACTION OF XYLARIA POLYMORPHA

LUAN Yang WEN Hua-An (Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Academia Sinica, Beijing 100080)  
Xylaria polymorpha was fermented in 80mL liquid medium of 4% glucose and 1% peptone in 250mL shake flask at pH6.5, 25℃ for 168 hours. Orthogonal experiments were designed to determine the suitable concentration of metal ions. The results indicated that 0.15% K+,0.03% Mg2+, 0.05% Ca2+, 0.0015% Zn2+, 0.001% Fe2+, 0.00005% Mn2+, 0.0002% Cu2+ were optimum. In 250L batch reactor, pH value was measured at intervals of 4 hours, deoxidize sugar was recorded at a 12 h interval and dissolved oxygen level was kept above 40%. Fermentative determination is at the 75th hour. Water- and alkali-extracted methods were used for extration of polysaccharides from the mycelium. The fermented solution was precipitated with alcohol and CTAB and proteins were thrown away with Sevag method. The remains were dissolved with NaCl solvent and dialysed with water. The result is that two kinds of polysaccharides, X-1 ( the water-soluble component ) and X-2 ( the alkali-soluble component ) , were gained. Their dry weight was recorded. Polysaccharide X-3 was also obtained in the fermented solution. Each of polysaccharides was proved to be homogeneous after examination by using chromatography on Sephadex G-100 and Sephadex G-150 columns, PAGE and paper chromatography. The polysaccharides include 16 amino acids. Their structures were analysized by I. R. spectrum and 1H-NMR spectrum.
【CateGory Index】: TQ461
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