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Applied studies on selection of high quality Pleurotus djamor strains by protoplast technology

WANG Qian1 LIU Yu-Xia1 ZHANG Yuan2 WANG Shi-Kui3 QI Ling-Di1 (1College of Life Sciences, Hebei University, Baoding 071002; 2Department of Biology, Baoding Teachers College, Baoding 071051; 3Chinese Academy of Agricultural Engineering,Beijing 100026)  
The preparation and regeneration of protoplast from Pleurotus djamor and the screening of protoplast regenerating strains were studied. Under the conditions as 30℃ 3h enzymdysis, the number of protoplasts of P. djamor mycelial aged 5 d could reach 8.4×107/mL. The highest regeneration rate of protoplast was 6.9% in the No.1 medium . Obtaining from the screening of protoplast regenerating strains, the strains H120 and H247 showed significantly rapid growth rate as compared with the original strain. The biological efficiency of H120 and H247 (F3 generation) were higher than that of original strain at significant difference level.Esterase isoenzyme analysis results showed that the variation existed among H120, H247 and original strain in zymogram of esterase isoenzyme
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