Biological characteristics and AFLP fingerprints of Phytophthora infestans strains DK98-1,DX98-2 and DX98-3
ZHU Jie-Hua YANG Zhi-Hui LIU Da-Qun LIU Shun (College of Plant Protection,Agricultural University of Hebei,Biological Control Center of Plant Disease and Plant Pests of Hebei Province,Baoding 071001,China)
Biological characteristics of the Phytophthora infestans strains DK98-1,DX98-2 and DX98-3 have been studied on the rye medium and V8-vegetable juice medium. As compared with the common strains,the colony growth of these strains was slow,the quantity of sporangia was few,and the quantity of oospore(2047～ 75623/cm2) produced was much more after sexual hybridization. DNA fingerprints of the 3 strains were detected by AFLP molecular marker. Strains DK98-1,DX98-2 and DX98-3 lack one band as compared with common strains on site of 330bp after they were amplified with the primer E+CG/M+CC,while they increase one band on site of 370bp after they were amplified with the primer E+AC/M+CT. It is indicated that these three strains distinguish from common strains at the genetic background. The frequency of the specific P. infestans strains could be investigated in nature by using these 2 specific primers.