Identification of sporulation-related genes in Aspergillus chevalieri var. intermedius by suppression subtractive hybridization
TAN Yu-Mei 1, 2 WANG Hai 1, 2 LIU Yong-Xiang 2 LIU Zuo-Yi 3 1 College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430000, China 2 Guizhou Institute of Biotechnology, Guiyang, Guizhou 550006, China 3 Guizhou Academy of Agricultural Science, Guiyang, Guizhou 550006, China
Aspergillus chevalieri var. intermedius was the dominant fermentative fungus in ‘fuzhuan’ tea. This fungus formed different kinds of spores under different osmotic pressure. The ascospore and conidia produced under low and high osmotic pressure, respectively, were used to construct suppression subtractive hybridization library with the vegetative mycelia as the control. Four hundred clones were selected from each library for nucleotide sequencing and the obtained sequences were searched against the GenBank database using the BLASTn and BLASTx programs. The differentiallyexpressed genes are related to protein metabolism, cell metabolism and transcriptional regulation, and so on. Among them, thirteen genes possibly related to sexual sporulation and ten genes possibly related to asexual sporulation were found.
【CateGory Index】： TS272.2