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《Fine Chemicals》 2009-06
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Antioxidant Activity of Extracts from Polygonum viscosum Buch.-Han.ex D.Don

CHANG Xing,WEI Jin-feng,LIU Yu-xin,KANG Wen-yi(Institute of Natural Products,Henan University,Kaifeng 475004,Henan,China)  
The total antioxidant activity of the extracts from Polygonum viscosum Buch.-Han.ex D.Don was assayed by three methods,namely,1,1-diphenyl-2-picryl-2-picrylhydrazyl(DPPH) radical scavenging,(2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS) radical scavenging,and ferric reducing antioxidant power(FRAP) assay.The results were compared with 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid(Trolox) and positive control butylated hydroxyanisole(BHA),2,6-di-tert-butyl-4-methylphenol(BHT).The results showed that P.viscosum Buch.-Han.ex D.Don had a good total antioxidant activity in vitro.The ethyl acetate extract from P.viscosum Buch.-Han.ex D.Don showed DPPH radical scavenging activity with IC50value of 7.89 mg/L higher than that of positive control BHT with IC50value of 18.71 mg/L,and slightly lower than that of BHA with IC50value of 3.20 mg/L.It showed ABTS radical scavenging activity with IC50value of 6.67 mg/L,higher than that of BHT with IC50value of 7.72 mg/L,and lower than that of BHA with IC50 value of 1.88 mg/L.It exhibited the ferric reducing antioxidant power with FRAP value of(1362.55±47.22) μmol TE/g,slightly lower than that of BHT with FRAP value of(1581.68±97.41) μmol TE/g.Among the three extracts of P.viscosum Buch.-Han.ex D.Don,the ethyl acetate extract showed the highest antioxidant activity,which was higher than the methanol extract.The results among the three methods were consistent,and the values obtained by ABTS assay were correlated the best with those obtained by DPPH assay(R=0.993).
【Fund】: 河南省教育厅基础研究计划(2008A360002);; 河南省教育厅青年骨干教师资助计划(2008)~~
【CateGory Index】: R285
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