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《Fine Chemicals》 2011-12
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Preparation of L-Rhamnose by Two-step Biotransformation of Naringin

WEI Sheng-hua,MENG Na,LI Wan-zhen,TAO Yu-gui (College of Biological and Chemical Engineering,Anhui Polytechnic University,Wuhu 241000,Anhui,China)  
The preparation of L-rhamnose by two-step biotransformation of naringin using naringinase and rest cells of yeast as catalyst was studied in this paper.The optimum conditions of hydrolysis were as follows: the mass concentration of naringin at 14 g/L,10 mL/L of naringinase,pH at 5.5,and reaction time of 22 h.The optimum conditions of fermentation were: the yeast dosage 2.5 g/L,the temperature at 32 ℃,pH at 6.0,and fermentation time of 120 min.A small-scale pilot was carried out in 30 L fermentor,and 85.6 g crystals of rhamnose was obtained,the purity of which was more than 98.5%.This yield accounted for 86.5% of the theoretical yield.
【CateGory Index】: TS264.9
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