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《Fine and Specialty Chemicals》 2015-05
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Study on the immobilization of nitrilase by LX-1000EP(C) epoxy resin

JI Dong-qing;ZHENG Ren-chao;ZHENG Yu-guo;Institute of Bioengineering,Zhejiang University of Technology;  
The free enzyme is difficult to recovery and repeated use,but the immobilized enzymes have high efficiency of repeating use.Optimial conditions of the immobilization of nitrilase by LX-1000EP(C)epoxy resin and the stability have been studied.The experimental results indicate that the optimal conditions of nitrilase immobilization are:the appropriate temperature is 20℃,the buffer used in immobilization process selects potassium phosphate buffer,the pH value is 8.0,the concentration of the buffer is 0.1mol/L,the amount of enzyme is 10 mL crude enzyme solution per gram of support.The recovery of the enzyme activity of the immobilized nitrilase reached98.1% under the conditions mentioned above.The remained activity is more than 30% of the initial enzyme activity after the immobilized enzyme is used repeatedly for 6times.
【CateGory Index】: Q946.5
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