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《Experimental and Laboratory Medicine》 2008-04
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Study on the genentic polymorphism of 17 Y-chromosome specific STR loci of non-related male individuals in southern Chinese Han population

Chen Yuekang, Li Qian, LI Dacheng, et al. Shenzhen Blood Center, Shenzhen 518035, China  
Objective To study the genetic polymorphism of the AmpFlSTR Y filerTM PCR amplification kit containing 17 Y-STR loci in southern Chinese Han non-related male individuals for medicalegal expertise. Methods A total of 119 cases of blood samples from southern Chinese Han non-related male individuals were subjected to Y-STR genotyping at 17 loci using the AmpFlSTR Y filerTM PCR amplification kit. 17 Y-STR loci were amplified in single multiplex and the PCR products were detected by using ABI PrismTM 3100 DNA Se- quencer. The frequency for each Y-STR haplotype and the haplotype diversity were calculat- ed. Results In the 119 unrelated male individuals, a total of 118 haplotypes at17 Y-STR loci were found and 117 haplotypes were observed only once, one haplotype was observed twice, the haplotype diversity was 0.9999. Conclusion Our results indicated that AmpFlSTR Y filerTM kit which allowing simultaneous analysis of 17 Y-STR loci is highly polymorphic and offers a high power of discrimination, which shows a broad application in personal identi- fication and paternity testing.
【Fund】: 深圳市科技计划项目(编号200701014)
【CateGory Index】: R394-33
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