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《Acta Agriculturae Universitatis Jiangxiensis》 2006-04
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A Study on Immunity of PRRS's Anti GP5 and M Protein Anti-antibody and Preparation

GUAN Yuan-hong,ZHANG Jing-jing,CHEN Zhen,XIE Bao-dong,ZHOU Zuo-hong~*(College of Animal Science and Technology,JAU,Nanchang 330045,China)  
This work included prepared PRRSV's antiantibody(Ab_2),discussed the modulating impact to organism immunity of Ab_2 in differ animal.Using purified anti PRRSV-GP5(GP5-Ab_1) and anti PRRSV-M(M-Ab_1) protein antibody to immunize Haerbin's big white rabbit,detecting the value,when the value achieved more than 1∶16,after another intensified immunity,blooding from heart,separating and purifying the rabbit anti antibody PRRSV-GP5IgG(GP5-Ab_2) and PRRSV-MIgG(M-Ab_2) serum,using GP5-Ab_2,M-Ab_2,the mixture of GP5-Ab_2 and M-Ab_2 immunized Kunming mice and no-immune with PRRS vaccine's piglet,set PRRS weaken-virulence vaccine and set the blank as the controls,the 21th day after immunity,blooding serum,the indirect ELISA detection results showed the kun-ming mice and piglet's serum immunity with Ab_2 and PRRS vaccine were all positive,the controls were negative.The results showed that Ab_2 in vivo had the function of replacing the PRRSV induced the differ organism mmunity,and these take a new way of the organism to avoid PRRSV's infection.
【Fund】: 国家“十五”重大科技专项(2001BA804A22)
【CateGory Index】: S852.4
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