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《Acta Agriculturae Universitatis Jiangxiensis》 2009-04
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Establishment and Optimization of AFLP Technological System for Hemibarbus labeo Pallas

TONG Guang-xing,KUANG You-yi,YIN Jia-sheng,XU Wei(Heilongjiang River Fishers Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China)  
An AFLP(amplified fragment length polymorphism) analysis system was established with Hemibarbus labeo Pallas by optimizing some factors of the AFLP technical system.The result show that optimizing the quality of template DNA,the concentration of two groups restriction enzymes,the time of the digested reaction and times of pre-amplification products dilution et al can obtained clear and stable amplification patterns.Compared results from two groups of restriction enzymes showed that the group of PstI and TaqI obtained more polymorphic bands than the group of EcoRI and Tru9I.This results provides fundamentals for further molecular studies of using AFLP marker.
【Fund】: 黑龙江省科委重点研究项目(GC05B509);; 黑龙江水产研究所基本科研业务费专项资助项目
【CateGory Index】: Q78
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