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《ACTA AGRICULTURAE UNIVERSITIS JIANGXIENSIS》 1998-02
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Genomic DNA Extraction and RAPD Analysis of Hot Pepper

Wang Deyuan1 Peng Shiqing2 Liu Zhixin2 Zheng Xueqin2(1 Institute of Vegetable Crops,Guangdong Academy of Agricultural Sciences,GuangZhou 510640; 2 National Key Biotech Lab for Tropical Crops,Danzhou,Hainan 571737)  
The preparation of DNA samples with good quality is the basis for researches in plant molecular biology.The DNA samples was prepared from hot pepper variety“Baojialia”and sweet pepper variety“Qiemen”by liquid N2-SDS method,SDS method and Benzyl Chloride method,respectively.The results showed that DNA samples,obtained by liquid N2-SDS method,possessed the ultraviolet absorbance spectrum of the pure natural DNA,and the A260/A280 was 1.60～1.70,A260 /A230 was 1.5～1.8.The yield of 60～90μg DNA per 100mg young leaves was obtained by liquid N2-SDS method.Also,the DNA samples prepared by this procedure were suitable for RAPD analysis,those isolated by other methods were unfit.Some steps were modified in liquid N2-SDS method because the degradation of RNA in DNA samples could affect the RAPD patterns.
【Key Words】： hot pepper DNA extraction RAPD analysis
【Fund】： 热带作物生物技术国家重点实验室开放课题
【CateGory Index】： Q946-33,S641.3
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【References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ji-yu~1,YUAN Qing-hua~2,ZHANG Wen-shu~2,LU Ting~3 (1. College of Pastoral Agriculture Science and Technology of Lanzhou University, Gansu Grassland Ecological Research Institute, Lanzhou 730020 China;2.Institute of Animal Science, CAAS,Beijing 100094 China; 3.Grassland College of Gansu Agriculture University, Lanzhou 730070, China);Genomic DNA Extraction and Optimizing RAPD Procedure of Lespedeza floribunda[J];Acta Agrestia Sinica;2004-03
2 DU Xiao-hua~(1,2),GONG Zhen-hui~1,WANG De-yuan~(2*),YIN Qiu-miao~2(1 College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Vegetable Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China);Genetic Differences among Well-performing Inbred Lines of Hot Pepper(Capsicum annuum L.)[J];Acta Botanica Boreali-Occidentalia Sinica;2006-12
3 WEN Qing-fang, ZHU Hai-sheng, LIN Yi-zhang, KANG Jian-ban, XUE Zhu-zheng (Institution of Cultivation and Rotation, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Genetic diversity of Lycopersicon esculentum var. cerasiforme germplasms by RAPD analysis[J];Fujian Journal of Agricultural Sciences;2006-01
4 WANG De-yuan(Vegetable Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Biotech Lab for Fruits and Vegetables,Guangzhou 510640,China);Primary studies on the genetic differences between F_1 and its parent lines in hot pepper(Capsicum annuum L.)[J];Guangdong Agricultural Sciences;2006-01
5 WANG De-yuan 1 WANG Ming 2 ZHENG Xue-qin 3(1.Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou, Guangdong, 510640; 2.College of Horticulture, Northwest Agri-forest Sci-Tech University, Yangling, Shaanxi,712100; 3.National Key Biotechnology Laboratory for Tropical Crops, Haikou, Hainan,571000);RAPD ANALYSIS ASSOCIATED WITH CYTOPLASM MALE STERILITY IN HOT PEPPER(Capsicum annuum L.)[J];Acta Agriculturae Nucleatae Sinica;2005-02
6 ZHANG Ning,WANG Feng shan (Institute of Biochemic and Biotechnological Drugs,School of Pharmacy , Shandong University,Jinan 250012, China);Advances of DNA extraction methods[J];Chinese Journal of Marine Drugs;2004-02
7 Hu Yongsheng1 Mao Shengli2 Liu Mingyue1 Zhang Baoxi2(1Horticulture and Garden College,Hunan Agricultural University,Changsha 410128) (2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences, Beijing 100081);Optimization of RAPD System for Studying the Resistance to Phytophthora Blight in Hot Pepper[J];Journal of China Capsicum;2007-01
8 NI Sui1,2,3,TIAN Min3,LI Ji-yuan3(1.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China;2.Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China;3.Research Institute of Subtropical Forestry,CAF,Fuyang 311400,China);A DNA Extraction Method for Sect. Camellia Gene Group[J];Journal of Ningbo University(Natural Science & Engineering Edition);2007-02
9 Ren Yu1 Zhang Yindong2 Yin Junmei1 Wang Deyuan3 1 Tropical Crops Genetic Resources Institute,CATAS Danzhou Hainan 571737 2 State Key Laboratory for Biotechnology of Tropical Crops,CATAS Haikou 571101 3 Vegetable Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Biotechnology Laboratory for Fruits and Vegetables Guangzhou 510640;SRAP Assessment of Genetic Relationship of Inbred Hot Pepper (Capsicum annuum L)[J];Chinese Journal of Tropical Crops;2008-01
10 QIAN Zhong-ying 1,2 ,CAI Run 1 ,PAN Jun-song 1 ,DONG Yi 1 (1.School of Agriculture and Biology,Shanghai Jiaotong University,Shang hai 201101;2.School of Life and Environmental Science,Shanghai Normal University,Shanghai 200234,China);Optimization and Application of RAPD System for Cucumber[J];Journal of Shanghai Jiaotong University (Agricultural Science);2003-03
China Proceedings of conference Full-text Database 2 Hits
1 Wang Deyuan (Vegetable Institute,Guangdong Academy of Agricultural Sciences,Guangdong Key Biotech Lab for Fruits and Vegetables,Guangzhou 510640);Primary Studies on the Genetic Differences between F_1 and its Parent Lines in Hot Pepper ( Capsicum annuum L.)[A];[C];2004
2 MA Lihua XIE Bing HUANG Minsheng (Dept. of Environmental Science, East China Normal University, Shanghai, 200062);The Study of DNA Extraction and Randomly Amplified Polymorphic DNA (RAPD) of White Rot Fungi[A];[C];2003
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Zi-ying(Department of Biology,Anhui Institute of Education,Hefei,230061,China);The Comparison of ISSR and PAPD Analysis of Edible Wild Mushrooms of Anhui[J];Journal of Anhui Institute of Education;2005-06
2 LIU Chun-xiang 1,SUN Bao-juan 2,ZHANG Han-qing 2 (1 Hoticultural Department of Shandong Agricultural University,Taian 271018,China; 2 Jilin Agricultural University,Changchun 130118 ,China );Study on heterosis of characters in eggplant F 1 hybrid[J];Shandong Agricultural Sciences;2001-03
3 Zhang Peijiang et el(Anhui Academy of Agricultural Sciences,Key Laboratory of Rice Genetics & Breeding,Ministry of Agricultural,Hefei 230031);Genetic Diversity Detected with RAPD Markers Among Rice Cultivars(Oryza sativa L.) and Its Utilizaton in Hybrid Rice Breeding[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1999-05
4 Zhang Peijiang et al (Anhui Academy of Agricultural Sciences, Key Laboratory of Rice Genetics & Breeding Ministry of Agricultural, The P. R. China, Hefei 230031);Genetic Diversity Detected with RFLP Markers among Rice Cultivars (Oryza Sativa L.) and Its Utilizaton in Hybrid Rice Breeding[J];Journal of Anhui Agricultural Sciences;2001-01
5 WANG Yun et al(Special Vegetable Research Institute,Hunan University of Arts and Science,Changde,Hunan 415000);Effects of the Different Fertilization Levels on the Growth and Development,and Prophase Yield of Dolichos lablab L.[J];Journal of Anhui Agricultural Sciences;2007-21
6 Liu Liwang et al(Department of Horticulture,Nanjing Agricultural University,210095);Genomic DNA Extraction and RAPD Analysis of Eggplant and Its Wild Relatives[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1997-03
7 WANG Xian-yuan, JIN Hong, XU Zhi-qin, GAO Lan-xing (Institute of hygiene and environmental medicine, Academy of military and medical sciences, 300050 Tianjin);Study on Hypoglycemic Effect of Momordica saponins and Its Mechanism[J];Amino Acids & Biotic Resources;2001-03
8 Zhu Li Wu Liu Tong Guang (Depanment of Horticulture, Anhui Agricultural College, Hefei, 230036);Study On The Peroxidase Isozymes Of Watermelon Hybrids And Their Parents[J];Journal of Anhui Agricultural University;1992-04
9 LI Pei jin,ZHU Su wen,CHENG Bei jiu (Bioengineering Department of Anhui Agricultural University,Hefei 230036);Study on Influencing Factors of RAPD in Maize[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2000-03
10 CHEN Rui-hong~1,GONG Zhen-hui~2(1.Department of Agricultural Engineering and Food Technology,Huaiyin Institute of Technology,Huaian 223001;2.College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry);Gray Correlative Degree Analysis of Main Quantitative Traits of Pepper[J];Journal of Anhui Agricultural University;2003-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LI Gang et al(College of Agronomy,Henan University of Science and Technology,Luoyang,Henan 471003);Study on DNA Extraction Methods of Paeonia suffruticosa and Paeonia lactiflora Pall[J];Journal of Anhui Agricultural Sciences;2007-32
2 YAN Miao-miao et al(Yantai Campus of Binzhou Medical College,Yantai,Shandong 264003);A Method Suitable for Extracting Genomic DNA from Animal and Plant——Modified CTAB Method[J];Journal of Anhui Agricultural Sciences;2008-20
3 DU Xiao-hua et al(Henan Institute of Science and Technology,Xinxiang,Henan 453003);Cluster Analysis of Chinese Pumpkin Inbred Lines[J];Journal of Anhui Agricultural Sciences;2008-32
4 ZHAO Jin-feng et al(College of Life Sciences,Nanjing Normal University,Nanjing,Jiangsu 210046);Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China[J];Journal of Anhui Agricultural Sciences;2008-33
5 TANG Li-bo et al(Department of Biology Engineering,Zunyi Medical College Zhuhai Campus,Zhuhai,Guangdong 519043);Genetic Diversity Analysis and Optimization of RAPD Reaction System of Michelia[J];Journal of Anhui Agricultural Sciences;2009-01
6 LIN Zhi-hua et al(College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225);Study on Extraction of Actinidia Genomic DNA[J];Journal of Anhui Agricultural Sciences;2009-03
7 ZHANG Tao(College of Life Science,Qingdao Agricultural University,Qingdao,Shandong 266109);Optimization on the Extraction Method of Genomic DNA from Anthurium andraeanum[J];Journal of Anhui Agricultural Sciences;2009-19
8 YANG Pei-hao et al(College of Life Science,Anhui Agricultural University,Hefei,Anhui 230036);Comparative Study on Extraction of Genomic DNA from Different Parts of Wheat[J];Journal of Anhui Agricultural Sciences;2009-22
9 LI Hai-bo(Yingdong College of Bioengineering,Shaoguan University,Shaoguan,Guangdong 512005);Comparative Study of Methods in Isolation Total DNA From Foeniculum vulgare Mill.[J];Northern Horticulture;2008-03
10 WANG Zan~(1,2),GAO Hong-wen~2 ,HAN Jian-guo~1(1.Institute of Grassland Science, China Agricultural University, Beijing 100094,China;2.Institute of Animal Science, Chinese Academy of Agricultural Science , Beijing 100094,China);DNA Extraction Method and Profile of AFLP Fingerprint of Caragana korshinskii[J];Acta Agrestia Sinica;2005-02
China Proceedings of conference Full-text Database 3 Hits
1 Zhang Zixue Hou Xilin (National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agric Univ,Nanjing 210095;Anhui Technical Teacher College,Fengyang 233100);Application of Regressive Analysis on Optimization of RAPD Reaction System in Pepper[A];[C];2004
2 Wang Deyuan (Vegetable Institute,Guangdong Academy of Agricultural Sciences,Guangdong Key Biotech Lab for Fruits and Vegetables,Guangzhou 510640);Primary Studies on the Genetic Differences between F_1 and its Parent Lines in Hot Pepper ( Capsicum annuum L.)[A];[C];2004
3 Zhouhui, Zhao fukuan, Lin cheng , Gaoxiahong, Cheng jihong (Beijing Agricultural College, Beijing 102206; Xinjiang Agricultural University ,Urumqi 830052,china);Optimization of RAPD analysis system in squash[A];[C];2004
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