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《Acta Agriculturae Jiangxi》 2008-10
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Extraction of Genomic DNA from Mature Leaves of Some Varieties of Prunus simonii

ZHANG Hui-rong1,QIAO Yu-shan2,CAO Shang-yin3,ZHANG Chuan-lai1,HU Hui-ling1,XUE Hu-bai3(1.Henan Institute of Science and Technology,Xinxiang 453003,China;2.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;3.Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China)  
The author have used the improved CTAB method to extract successfully the genomic DNA from mature leaves of some varieties of Prunus simonii.Firstly,before caryons lysis the washing buffer was used to avoid interference from amylose,polyphenol,tannin,pectin and so on in the cytoplasm;secondly,during caryons lysis,alcohol was mixed into the extraction buffer to further eliminate amylase.The results showed that the extracted DNA from the mature leaves by this method was pure and integral,the value of OD260nm/OD280nm was from 1.70 to 1.93,the degradation of extracted DNA was not found,the amylose in DNA was eliminated completely,and the DNA was suitable for digestion by restrictive endonucleases.SRAP analysis indicated the primer(me1/em1) produced clear polymorphic patterns.Therefore this method could be used to extract ideal DNA samples from the mature leaves of Prunus simonii Carr.and was suitable for PCR and restrictive enzyme analysis.
【Fund】: 河南科技学院重点科研项目(06029)
【CateGory Index】: S662.3
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