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Evolution of Calcium- dependent Protein Kinase Gene Family in Apple( Malus domestica)

CHEN Fei;YIN Huan;LIANG Ying-hai;CAI Bin;Laboratory of Fruit Systematical Biology,Nanjing Agricultural University;  
The 26 characterized Calcium-dependent Protein Kinase(CDPK) genes were encoded from the genome of apple(Malus domestica). The similarities and differences among CDPK members were acquired by comparison of gene structure and domain organization. Through the analysis of third-class structure of protein,the mutation of apple CDPK at key locus was obtained,which might cause major differences in auto-inhibition and activation of CDPK structure. The phylogenetic tree of the members of apple CDPK was analyzed,which got the origin of the 26 CDPKs and 21 amplifications. The preliminary expression of CDPK in apple was understood by EST data. The complex evolutionary history of perfect apple CDPK members laid the foundation on carrying out the validation of functional contact and gene utilization.
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