Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Integrated Traditional and Western Nephrology》 2012-11
Add to Favorite Get Latest Update

The Effect of Utility Monomer in Epimedium to the Renal Fibroblast Cell Line and Mesangial Cell Line

WANG Dong,ZHANG Jiang,WU Tongru,et al Shuguang Hospital,Shanghai University of TCM,Shanghai(200021)  
Objective:To observe the effect of utility monomer in Epimedium to the renal fibroblast cell line and mesangial cell line.Methods:To culture in vitro the renal fibroblast cell line and mesangial cell line,were divided into blank control group,TGF-β1 2 ng/ml group,TGF-β1 2 ng/ml+Chinese medicine monomer 10-5 g/ml group and TGF-β1 2 ng/ml + Chinese medicine monomer 10-6 g/ml group.To observe the effect of Chinese medicine monomer for 24 hours the two cell lines by MTT assay,and the secretion of TGF-β by ELISA assay.Results:Compared with blank control group,adding TGF-β1-stimulating factor can promote the two cell lines (P0.05),as well as the secretion of TGF-β (P0.01),Chinese medicine monomer can inhibit the activation of two cell lines proliferation and secretion of TGF-β (P0.05 or P0.01).Conclusion:Utility monomer of Epimedium can inhibit the activation of the renal fibroblast cell line and mesangial cell line proliferation and secretion of TGF-β,effective prevention and treatment of renal fibrosis,and its possible mechanism of action relate to inhibition of TGF-β secretion.
【Key Words】: fibroblast line intercapillary cell line culture in vitro Epimedium Chinese medicine monomer transforming growth factor-β
【Fund】: 国家科技重大专项项目(No.2009ZX09311-003);; 国家自然基金资助项目(No.81173219);; 科技部中医药行业科研专项项目(No.201007005);; 高等学校博士学科点专项科研基金项目(No.20093107110006);; 上海市科委创新行动计划项目(No.11DZ1973100);; 上海市教育委员会E-研究院建设计划资助项目(No.E03008);; 上海高校创新团队建设项目(No.200908)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yao,YU Li,ZHANG Lei,ZHANG Jian-qing.Department of Pediatrics,The Affiliated Guangzhou First Municipal Hospital of Guangzhou Medical College,Guangzhou 510180,China;Expression and implication of TGF-β1 and CTGF in rat glomerulosclerosis model[J];Guangdong Medical Journal;2010-06
2 ZHOU Le,CUI Liao,WU Tie(Department of Pharmacology,Guangdong Medical College,Dongguan,523808,P.R.China);Effect of herba epimedii on renal bone morphogenetic protein-7 in male rats with kidney-YANG insufficiency[J];Journal of Guangdong Medical College;2007-04
3 CHENG Hong,SONG Enfeng Renmin Hospital of Wuhan University,Wuhan (430060);Effects of Eucommia on TGF-β_1/Smad Signaling Pathway in Renal Interstitial Fibrosis in Rats with Unilateral Ureteral Obstruction[J];Chinese Journal of Integrated Traditional and Western Nephrology;2009-06
4 CEN Jie1 WANG Yi2 1. Yueyang Clinical College of Shanghai University of Traditional Chinese Medicine 2. Yueyang Hospital of Integrative Chinese & Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine;Comparison Among Cortex Cinnamomi, Radix Aconiti Preaeparata and Herba Epimedii in Treating Chronic Renal Failure[J];Shanghai Journal of Traditional Chinese Medicine;2007-12
5 JIA Liang-liang, YUAN Ding, WANG Hong-wu, HE Yu-min, ZHANG Chang-cheng (Medical College of China Three Gorges University, Yichang 443002, China);The Pharmacological Research Advance of Icariin[J];Progress in Modern Biomedicine;2010-20
6 HU Peng,QIN Yuan-han,JING Cheng-xue(Department of Pediatrics,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China);Transforming Growth Factor-β and Phenotypic Transdifferentiation of Renal Residential Cells[J];Journal of Applied Clinical Pediatrics;2008-05
7 Yang Chen,Jian-hua Huang,You Ning,Zi-yin Shen Institute of Integrated Chinese and Western Medicine,Huashan Hospital,Fudan University,Shanghai 200040,China;Icariin and its pharmaceutical efficacy:research progress of molecular mechanism[J];Journal of Chinese Integrative Medicine;2011-11
8 LI Zuo-zhou, XU Yan-qin, WANG Ying, HUANG Hong-wen(Wuhan Botany Garden/Wuhan Institute of Botany, Chinese Academy of Sciences; Wuhan 430074, China);Status and prospect of research on medicinal plants of Epimedium L.[J];Chinese Traditional and Herbal Drugs;2005-02
9 ZHANG Hua-feng1,YANG Xiao-hua2(1.National Engineering Laboratory of Endangered Medicinal Materials Resources Development in Northwest China,College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi′an 710062,China;2.School of Medicine,Xi′an Jiaotong University,Xi′an 710049,China);Bioactive constituents in Herba Epimedii and their developmental strategies[J];Chinese Traditional and Herbal Drugs;2010-02
10 LI Li,WANG Xue-mei(Department of Integrated Chinese and Western Medicine,First Hospital,Peking University,Beijing 100034,China);Progress of pharmacological research on icariin[J];China Journal of Chinese Materia Medica;2008-23
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Qing-jie et al(Jilin Tianyao Technology Co.,Ltd,Changchun,Jilin 130012);Study on Extraction of Compound Luronggubao Capsule[J];Journal of Anhui Agricultural Sciences;2011-12
2 ZHAO Yan-hong1,2 JIA Xiao-bin1,2 CHEN Yan1 Ming Hu3(1Jiangsu Academy of Chinese Medicine Sciences,Jiangsu Engineering and Technology Research Center forModern Chinese Pharmaceutical Preparation,Nanjing 210028,China;2School of Pharmacy,Jiangsu University,Zhenjiang 212013,China;3University of Houston,U S A);Study on in situ intestinal absorption kinetics of icariin in rats[J];China Journal of Traditional Chinese Medicine and Pharmacy;2007-10
3 ZHANG Xin-zhi1,HUANG Di2,WU Feng1,HAO Jun-jie1,LI Hui-long3,HE Li-qun2 (1Department of Nephrology,Jinshan Branch of Shanghai No.6 Hospital,Shanghai 201500,China;2Department of Nephrology,Shuguang Hospital Affiliated to Shanghai Chinese Medical University,Shanghai 201203,China);Experimental study on role of TGF-β1/p38 mitogen-activated protein kinase pathway to renal interstitial fibrosis and intervention mechanisms of Kang Xianling Decoction[J];China Journal of Traditional Chinese Medicine and Pharmacy;2011-02
4 XIAO Hou-qin, ZHANG Jian-e, DING Guo-hua, et al. Department of Nephropathy, The Affiliated Taihe Hospital of Yunyang Medical College, Hubei 442000, China;Celecoxib Suppresses the Expression of Connective Tissue Growth Factor by up-regulating cAMP in Rats with Unilateral Ureteral Obstruction Model[J];Journal of Chinese Physician;2005-09
5 CHEN Hong-jie1,2,CAO Lei1,WANG Shou-sen1,ZHENG Zhao-cong1 1.Department of Neurosurgery,Fuzhou General Hospital,PLA Nanjing Military Area Command,Fuzhou 350025,Fujian,China 2.Clinical Medicine College,Fuzhou General Hospital,Fujian Medical University,Fuzhou 350025,Fujian,China;Effect of recombinant adenovirus livin siRNA on proliferation and apoptosis of U251 cells[J];Academic Journal of Second Military Medical University;2011-06
6 QUAN Qiu-mei,LI Yun-xiang,WU Chun-mei,ZENG Ran,GUO Qiang(Sichuan Provincial Key Laboratory of Environmental Science and Biodiversity Conservation,China West Normal University,Nanchong,Sichuan 637002,China);Pollen Germination and Growth of Epimedium brevicornum[J];Acta Botanica Boreali-Occidentalia Sinica;2007-10
7 ZHAO Ling,LAN Liu-gui,MIN Xiao-li,LU Ai-hua,ZHU Li-qun,HE Xiao-hong,HE Ling-jie Department of Endocrinology,Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China;Integrated treatment of traditional Chinese medicine and western medicine for early-and intermediate-stage diabetic nephropathy[J];Journal of Southern Medical University;2007-07
8 Yu Dan,Zhang Yanlin~*,Fang Ya~(**),et al.Department of Nuclear Medicine,Xiamen,Fujian(361003)~*Department of Nephropathy,First Hospital of Xiamen,Fujian Medical University~(**)()Department of Phylaxiology,Medical College of Xiamen University;Study on the Changes and Correlations of Plasma Adrenomedullin,Brain Natriuretic Peptide and TGF-β_1 Levels in the Patients with Chronic Renal Failure[J];Journal of Radioimmunology;2006-04
9 WEI Zhong-nan,ZHU Min,WU Qiang,et al(The Second Affiliated Hospital,FUTCM,Fuzhou,Fujian 350003,China);Clinical Observation of Supplementing Qi Nourishing Yin Tonghua Decoction in Treating 31 Cases of Albuminuria in Diabetic Nephropathy[J];Journal of Fujian University of Traditional Chinese Medicine;2010-01
10 LI Mu-dan,SHI Xu,GUAN Ping(College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China);Optimization of ITS-OCR Amplification Conditions of Epimedium acuminatum[J];Guizhou Agricultural Sciences;2009-08
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Li Baiqing, (Department of Microbiology, Bengbu Medical College, Anhui );EFFECTS OF CHINESE DRUGS INVIGORATING KIDNEY ON CELLULAR IMMUNITY I . Effect of Epimedium and Cistanche on E-rosette formation and activity of acid α——naphthyl acetate esterase in human lymphocyte[J];Journal of Bengbu Medical College;1985-03
2 SHEN Xiao-jun,WEI Yan,HE Hang(Henan University of TCM,Zhengzhou 450008,China);Effect of icariin on expression of glucose regulated protein78 in atherosclerosis tissue[J];China Journal of Traditional Chinese Medicine and Pharmacy;2010-05
3 WANG Hai-Yan △, LI Jing-Zi, PAN Ji-Sheng, ZOU Wan-Zhong, LI Xiao- Mei,ZHANG You-Kang, ZHU Shi-Le, HUANG Hai-Chang, WANG Shu-Xian (Institute of Nephrology, Peking University First Hospital, Beijing 100034,China);The effect of Astragali and Angelica on nephrotic syndrome and its mechanisms of action[J];Journal of Beijing Medical University;2002-05
4 LI Ling, ZOU Wanzhong △, ZHANG Bo,WANG Shenglan, GUO Hua (Department of Pathology,Peking University School of Basic Medical Sciences, Beijing 100083,China);The phenotypic transformation of renal tubular epithelial cells affected by pathological stimuli[J];Journal of Beijing Medical University;2003-01
5 HE Wei1,LI Zi-li1,CUI Yuan-lu2,YI Biao1,LIANG Cheng1,WANG Xiao-xia1,LI Yang1,WANG Xing1(1.Center of Orthognathic Surgery,Peking University School and Hospital of Stomatology,Beijing 100081,China;2.TCM Research Center,Tianjin University of Traditional Chinese Medicine);Effect of icariin on the mRNA expressions of Cbfα1,BMP2,BMP4 in rat osteoblasts[J];Journal of Peking University(Health Sciences);2009-06
6 FU Li-bo1,2,XU Hai-yan2,LIU Feng-lian3,YANG Yan-long1,YANG Yan-ping2(1.Basic Medical College,Jinlin University,Changchun 130021,China;2.College of Life Sciences,Changchun Teachers College,Changchun 130032,China;3.Experiment and Education Technology Center,Linyi Teachers College,Linyi 276005,China);Effects of epimedium on blood pressure in normal and stress-induced rats[J];Journal of Northeast Normal University(Natural Science Edition);2008-01
7 Li Shutong; Li Tiejun; Zheng Qinyue; Wang Hongbin (Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai,200433);Effects of icariine on lymphocyte proliferation and production of colony stimulating factor-like activity from mice splenocytes in vitro[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1995-04
8 Li Shutong;Li Tiejun;Zheng Qinyue;Wang Hongbin(Department of Pharmacology,College of Pharmacy,Second Military Medical University,Shanghai,200433);Effects of icarrin on function of murine peritoneal macrophages[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1995-06
9 Qin Luping, Shi Hanping, Zheng Shuiqing, Li Bohua, Hong Qiubing, Wang Hongbin, Zhang Jiaqing (Department of Pharmacognosy, College of Pharmacy,Second Military Medicial University, Shanghai, 200433);Effects of osthol and icariin on serum thyroid hormone in mice of hypothyroidism type kidneyyang insufficiency[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1998-01
10 LI Li~ 1 , ZHOU Qi-xin~ 1 , SHI Jing-shan~ 2 (~ 1 Research Center of Biochemistry and Molecular Pharmacology, Chongqing University of Medical Sciences, Chongqing 400016, ~ 2 Department of Pharmacology, Zunyi Medical College, Zunyi 563003, China);Effect of icariin on expression of cytochrome C oxidase subunit Ⅱ mRNA in mice of cerebral ischemia/reperfusion[J];Acta Academiae Medicinae Militaris Tertiae;2006-07
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved