Study of HIF-1α Expression in Mesangial Proliferative Glomerulonephritis Trends and MsPGN TCM Syndrome Type Correlation
SHAO Guomin,CHEN Xiaorong,ZHANG Shizhao Department of Nephrology,People's Hospital of Zhejiang City in Fuyang Province,Fuyang(311400)
Objective:Study of hypoxia inducible factor -1 (HIF-1) expression in mesangial proliferative glomerulonephritis trends and MsPGN TCM syndrome type correlation。Methods:A retrospective analysis of the hospital 's primary MsPGN 120 cases , divided into four types according to TCM . Leniently the patients with in severe MsPGN , renal biopsy tissue paraffin blocks , randomly selected 40 renal biopsy tissue samples , the application of the two-step immunohistochemical Envision detected with mild to severe MsPGN renal biopsy tissue samples glomerular and renal district HIF-1α expression changes , using a semi-quantitative analysis of HPIAS-2000 high definition color pathology image analysis system part of the relative area of specific staining of glomerular and tubular interstitial , and statistical analysis .Results:HIF-1α in mesangial proliferative glomerulonephritis in patients with renal biopsy tissue with mesangial proliferative lesions increase in glomerular renal tubular expression increase. TCM Syndrome Types in MsPGN , lung and kidney qi deficiency type of HIF-1α in glomerular expression of the other three types are different (P0.05); the Spleen deficiency in HIF-1α in glomerular expression of the other three types have a significant difference (P0.01 ) ; Yin Deficiency type of liver and kidney in HIF-1α expression in glomerular no significant difference (P0.05 ).Conclusion:HIF-1α with MsPGN severity ; the pathological type MsPGN patients may be a different card Hou , prompt treatment should pay attention to .