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Research of Pulmonary Artery Perfusion with Hypothermic Lung-protective Solution Inhibits Apoptosis in Lung Tissue Cell

YU Qiu-ping1,WAN Yu-hua2,LOU Li-ping3,ZHOU Xue-liang2,WAN Li2,YU Ben-tong2(1.ICU,the Affiliated Hospital of Jiujiang College,Jiujiang 332000,China;2.Department of Cardiovascular and Thoracic Surgery,the First AffiliatedHospital of Nanchang University,Nanchang 330006,China;3.Department ofInternal Medicine,the People's Hospital of Jiujiang,Jiujiang 332100,China)  
Objective To explore effects of pulmonary artery perfusion with hypothermic lung-protective solution on the apoptosis of lung tissue cells during cardiopulmonary bypass(CPB).Methods Twenty cases undergoing mitral valve replacement(MVR) were randomly divided into lung protective group and control group with 10 cases in each group.The patients were replaced mechanical mitral valve under CPB.Hypothermic lung-protective solution was infused to the main pulmonary artery in the lung protective group,and sodium chloride was infused to the main pulmonary artery in the control group.Lung biopsy specimens(1.0 cm×1.0 cm×1.0 cm) were obtained before and after CPB in order to study the apoptosis of lung tissue cells using terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling(TUNEL).At same time,1 mL artery blood was taken for blood gas analysis.Patients' lung functions and calculation oxygenation index were monitored after CPB 0,6,12,24 h.Results The oxygenation index was higher in the lung protective group than that in the control group at 0,6,12,24 h after CPB(P 0.01 or P0.05).In sitau Tunnel staining showed that after CPB,the number of apoptosis cell in lung protective group and control group increased significantly compared with those of before CPB of both(all P0.01).After CPB,the rate of apoptosis cells of lung tissue cells was(18.68±1.34)% in control group,whereas(10.46±1.96)% in lung protective group.There was significant difference between two groups(P0.01).Conclusion(1)Conventional hypothermic CPB induces apoptosis in lung tissue cells.(2)Pulmonary artery perfusion with hypothermic lung-protective solution can inhibit the apoptosis of lung tissue cells and relieve CPB induced lung injury,demonstrates the effects of lung protection.
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