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《Journal of Nanchang University(Medical Science)》 2010-03
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Study of Preconditioning with Polygona-Polysaccharose Protects Cultured Neonatal Rat Myocardial Cells against Anoxia/Reoxygenation Injury

ZHU Ye-fenga,LIU Ji-chunb,HE Mingc(a.2007 Grade of Medical Department of Graduate School;b.Department ofChest Surgery,the First Affiliated Hospital;c.Department of Pharmacology,Medical College,Nanchang University,Nanchang 330006,China)  
Objective To explore the protective effects of Polygona-Polysaccharose(PSP) on neonatal rat myocardial cells with anoxia/reoxygenation(A/R) injury and the mechanisms.Methods The myocardial cells were isolated from 20 SD neonatal rats and cultured,followed by random table method divided into four groups after being cultured for 48 hours(the experiment was duplicated 6 times in each groups),namely:normal control group,A/R group,anoxia preconditioning(APC)group(APC+A/R group),PSP preconditioning group(PSP+A/R group).Various techniques were adopted to detect the cells and cellular products:the levels of myocardial cells viability rate was assay by MTT colorimetric method;the levels of culture fluid of released lactate dehydrogenase(LDH),superoxide dismutase(SOD),and malondialde hyde(MAD);the ratio of myocardial cells apoptosis was detected by Annexin-V/PI assays with flow cytometry.Results Compared with the control group,the myocardial cells viability,the levels of culture fluid of released SOD of A/R group significantly decreased(all P0.01),whereas levels of culture fluid of released MDA,LDH,and the ratio of apoptosis significantly increased(all P0.01).Compared with A/R group,the myocardial cells viability and the levels of intracellular SOD of APC+A/R group and PSP+A/R group were significantly increased(all P0.01),while levels of culture fluid of released MDA,LDH,and the ratio of apoptosis significantly decreased(P0.01,respectively).Compared with APC+A/R group,the myocardial cells viability and the levels of intracellular SOD,MDA,LDH,and the ratio of apoptosis were not statistically significant(all P0.05).Conclusion It suggests that PSP could protect myocardial cells against A/R injury,the mechanisms of which lie in that PSP could increase activities of endogenous antioxidants.
【CateGory Index】: R285.5
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