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《中国人民解放军军医大学学报(英文版)》 1998-02
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Sequence differences of rDNA-ITS2 and species-diagnostic PCR assay of Anopheles sinensis and Anopheles anthropophagus from China

Ma Yajun Qu Fengyi , Xu Jiannong , Zheng Zheming(Department of Parasitology, Second Military Medical University, Shanghai 200433)(Zoological Institute, Life Sciences College of Shanxi Normal University, Xi'an 710062)  
Anopheles sinensis and An. anihropophagus are two morphologically indistinguishable yet genetically and behaviorally distinct mosquito species that vary dramatically in their importance as vectors of malaria inChina. The sequence of the ribosomal DNA internal transcribed spacer 2 (ITS2) was determined for bothspecies to assess the species differentiation. The lengths of ITS2 was 468 hp for An. sinensis and 452 hp forAn. anthropophagus. Interspecies difference in sequence was 28. 8%. Intraspecies sequence divergence wasnegligible. A PCR method was developed for distinguishing the two species based on the species-specific variations of the ITS2 sequences. The method would amplify a diagnostic fragment in length of 425 hp for An.sinensis and 253 hp for An. anthropophagus. Field collections from 12 localities in 10 provinces of China weretested. In 440 mosquitoes identified by adult morphological characteristics as An. sinensis, 291 (66. 2 % ) wereidentified later as An. sinensis and 56 (12. 7 % ) as An. anthropophagus, the remaining 93 (22. 1 % ) were notamplified by PCR. The results showed that the morphological characteritics of adult was not reliable for fieldidentification. The PCR assay was a simple, fast and reliable method for species identification.
【CateGory Index】: R394
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【References】
Chinese Journal Full-text Database 1 Hits
1 LI Mei,TANG Lin-hua. Department of Malaria, National Institute of Parasitic Disease, Chinese Center for Disease Control and Prevention, Shanghai 200025, China;Primary study on the difference of bacteria borne by two kinds of Anopheles mosquitoes[J];Chinese Journal of Vector Biology and Control;2008-04
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 Ma Yajun, Qu Fengyi, Xu Jiannong, Zheng Zhemin (Department of Parasitology, Department of Basic Medicine, Second Military Medical University, Shanghai, 200433);Differentiation of Anopheles sinensis and Anopheles anthropophagus using a ribosomal DNA PCR assay[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1998-03
2 Song Shewu Zhao Tongyan Jiang ShunanDepartment of Plant Protection,Southwest Agricultural University,Beibei,Chongqing 400716 Lu Baolin (Institute of Microbiology & Epidemiology,Beijing 100071);THE SEQUENCING AND SEQUENCES ANALYSES OF rDNA-ITS2 OF CULEX PIPIENS COMPLEX IN CHINA[J];Acta Parasitologica Et Medica Entomologica Sinica;2003-02
3 QU Feng-yi(Department of Etiological Biology,the Second Military Medical University,Shanghai 200433,China);Current Status,Utilization and Sharing of Mosquito Germplasm Resources in China[J];Chinese Journal of Parasitology and Parasitic Diseases;2006-S1
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 Liu Chaofan, Qian Huilin, Gu Zhengcheng, Chang Xiang, Wu Zhenyou, Chen Fuqun, Chen YuzuInstitute of Parasitic Diseases, China National Centre for Preventive Medicine Anhui Provincial Sanitary and Anti-epidemic Station;THE ROLE OF ANOPHELES LESTERI ANTHROPOPHAGUS IN MALARIA TRANSMISSION IN JIANGHUAI REGION, ANHUI[J];Chinese Journal of Parasitology and Parasitic Diseases;1984-04
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