Effect of monocyte chemoattractant protein-1 on chemotactic gene expression by macrophage cell line U937
BIAN Guang-xing 1,GUO Bao-yu 1**, MIAO Hong 1, QIU Lei 1, CAO Dong-mei 2, DAO Shu-yan 1, ZHANG Ran 1 1Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433,China 2Department of Pathology and Physiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
Objective: To study the chemotactic superfamily genes expression profiling of macrophage line U937 treated with monocyte chemoattractant protein-1 (MCP-1) using gene chip technique. Methods: Total RNA from macrophage line U937 (as control) and U937 with MCP-1 was extracted, made reverse transcript to cDNA and tested with gene expression chip HO2 human. Results: Some chemotactic-related gene expressions were changed in all analyzed genes. Regulated upon activation, normal T cell expressed and secreted (RANTES) was up-regulated over 2-fold and 7 chemotactic-related genes (CCR2, CCR5, CCL16, GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2) were down-regulated over 2-fold in MCP-1 treated U937 cells at mRNA level. Conclusion: MCP-1 can influence some chemokines and receptors expression in macrophage in vitro, in which MCP-1 mainly down-regulates the chemotactic genes expression of those influencing neutrophilic granulocyte (GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2). Another novel finding is that it can also down-regulate the mRNA level of CCR5, which plays a critical role in many disorders and illnesses.