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《Clinical Journal of Medical Officer》 2007-06
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Experience in Making One-kidney and One-clip Renovascular Hypertension Animal Model

Sun Qi1,Huang Rong-gui2,Zheng Xing-zhong2,Liang Meng1(1.Department of Nephrology,PLA No.174 Hospital,Xiamen 361003,China;2.Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China)  
Objective To study the preparation of one-kidney and one-clip(1K1C) renovascular hypertension animal model. Methods The animal models of 1K1C renovascular hypertension were established with Sprague-Dawley rats(200-220g per rat).The rats were randomly divided into four groups,i.e.normal group,one-kidney(1K) group,1K1C group and 1K1C+BEA group(BEA=2-bromoethylamine hydrobromide).The renal medulla was destroyed by BEA.Blood pressure was measured with tail-cuff method by rat blood pressure meter of type RBP-1.Results Compared with normal control group,the blood pressure in 1K group obviously increased(P0.05),but four weeks later the blood pressure in both the groups did not achieved the standard of renovascular hypertension.The blood pressure in 1K1C group began to increase one week later and achieved the threshold standard of renovascular hypertension two weeks later.Compared with 1K1C group the blood pressure in 1K1C+BEA group increased more obviously after four weeks(P0.05).Conclusion Our making method to rat model of renovascular hypertension is simple and easy to operate.The success rate is about 80%,similar with those in the previous reports.
【CateGory Index】: R-332
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