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《Academic Journal of Pla Postgraduate Medical School》 2004-06
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The measurement of reticulocytes and clinical applications in the elderly

YUE Jia-xin 1, CONG Yu-long 1, ZHOU Jian-shan 1, WANG Hai-hong 1,LAN Ya-ting 1, XU Da-tao 2, GU Yi-hai 3(1.Clinical Laboratory in PLA General Hospital,Beijing 100853,China; 2.Clinical Laboratory, PLA 401th Hospital, QingDao 266071, China; Clinical Laboratory, Hanzhong 3201th Hospital,Shanxi 723000,China)  
Objective:to establish a reference value of the reticulocytes counts and its subpopulation in the elderly, and study the value of its clinical applications in anemias diagnosis and leukemias chemotherapy.Methods:The reticulocytes were measured by automated hematology analyzer (ADVIA 120) with oxazine 750 as fluorochrome in 338 the healthy elderly and 141 aged hematological disorders. Results:The Student′s t-test showed that there were no significant differences(P0.05)between male and female in the healthy elderly for reticulocyte percentage (RET) , reticulocyte absolute number(RET#), High fluorescence reticulocyt(HFR),middle fluorescence reticulocytes(MFR) and low fluorescence reticulocytes (LFR). They were 0.0140± 0.0045,(62.22±19.28)×10 9/L、0.8571±0.0441、0.1190±0.0336 and 0.0239±0.0139。There were significant differences(P0.05)between anemias and reference value in the elderly for RET# and HFR+MFR. RET# and HFR+MFR was extremely decreased in leukemias after chemotherapy. HFR+MFR rise faster than RET# and the absolute neutrophil count (ANC)in bone marrow recovery. Conclusions:Reference value of the reticulocyte counts and its subpopulation were not correlated with sex in the healthy elderly. RET# and HFR+MFR have important significance in classification and difference diagnosis of anemias, estimation of bone marrow recovery after chemotherapy in the elderly. [
【CateGory Index】: R446.11
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