Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Laboratory Medicine and Clinic》 2009-15
Add to Favorite Get Latest Update

Choice of RhD negative detection methods in laboratories for healthy persons

DENG Xi,YANG Yu-ming,YI Shuang-qing.Blood Center in Xiaogan City,Hubei 432000,China  
Objective To probe into the laboratory resist to HCV,HIV,HBsAg,syphilis,ALT,and the resposivity in the sieving test of RhD blood type,and offer basis to establish the negative blood donor′s infectious disease of RhD,as well as the choice of the experimental method for the blood group.Methods 1.Collect 232279 copies of resist blood donors serum in Xiaogan district,and conduct the test to-HCV,resisting-HIV,HBsAg,syphilis by means of ELISA,and detect it further to confirm the positive sample spreads recording by going against enzymes together with(RT-PCR);2.Do test to the blood donors' ALT through hanging on law or speeding law;3.Utilize the little board law to sieve the blood group of RhD for the blood donors,and confirm the RhD negative samples with U.S.A.Dominion Biologcals Limited monoclonal antibody reagent.Results The rate of 232279 copies of blood final proof to resiste-HCV,-HIV,HBsAg,syphilis,ALT the positive is 1.52%(3540/232 279),0.004%(1/232 279),4.20%(9752/232 279),0.06%(137/232 279),and 2.72%(6321/232 279)respectively;The negative rate of the blood group of RhD after further sieving and confirming is 0.32%(746/232 279),and 0.30%(700/232 279)respectively.Conclusion The method of ELISA law and speed law to RhD negative infectious disease is of high sensitivity and picularity,and the result is accurate,so it can be regarded as the measuring experiment method of blood donor′s infectious disease for the patients.It has swift,sensitive,fruits characteristics,such as accuracy,economy,etc.so the little board law measures of the blood group of RhD is suitable for the blood group of RhD to sift and check on a large scale.
【Fund】: 湖北省卫生厅2008年度采供血管理专项科研项目(CGX2008-6)
【CateGory Index】: R446.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved